Schaberg D R, Haley R W, Terry P M, McGowan J E
J Clin Microbiol. 1977 Oct;6(4):359-61. doi: 10.1128/jcm.6.4.359-361.1977.
The direct immunofluorescence technique for detecting antibody-coated bacteria in urinary sediment is felt to be useful in distinguishing infection of the kidney from infection of the bladder. An independent, blind multiple-reading system was used to measure interobserver variability in the evaluation of slides of urinary sediments for antibody-coated bacteria. Three independent observers agreed unanimously on first reading in 88% of 253 specimens. When compared with the majority opinion, the sensitivity and specificity of an individual reading were 91 and 95%, respectively.
检测尿沉渣中抗体包被细菌的直接免疫荧光技术被认为有助于区分肾脏感染和膀胱感染。使用了一个独立的、盲法多次阅读系统来测量观察者之间在评估尿沉渣中抗体包被细菌玻片时的变异性。在253个标本中,88%的标本在首次阅读时三位独立观察者意见一致。与多数意见相比,单次阅读的敏感性和特异性分别为91%和95%。