Faral-Tello Paula, Greif Gonzalo, Satragno Dinora, Basmadjián Yester, Robello Carlos
Laboratorio de Interacciones Hospedero Patógeno , Unidad de Biología Molecular , Institut Pasteur de Montevideo , Mataojo 2020 , Montevideo , 11400 , Uruguay.
Centro Hospital Veterinario , Facultad de Veterinaria , Universidad de la República , Uruguay.
RSC Med Chem. 2020 Jul 6;11(8):913-918. doi: 10.1039/d0md00073f. eCollection 2020 Aug 1.
Leishmaniasis is a neglected disease caused by a protozoan parasite of the species in over 98 countries in five continents. Visceral leishmaniasis is one of the main forms of the disease and is mainly caused by , whose main vector is the dipteran The presence of the vector in Uruguay was recorded for the first time in 2010 and an autochthonous outbreak of canine visceral leishmaniasis occurred in the northern locality of the country in 2015. We report the isolation in blood-free FBS-supplemented defined media of five isolates responsible for the referred outbreak, and characterize them in terms of their growth as promastigotes, infectivity and replication in human derived monocytes and drug resistance. Results indicate similar promastigote growth among the strains, enhanced infectivity and replication for the five strains isolated from the Uruguayan outbreak when compared with reference strains from South America, equivalent drug susceptibility for miltefosine and nifurtimox and a significant difference in IC values for amphotericin B between the Uruguayan strains, 3-4 fold higher than the reference strain.
利什曼病是一种被忽视的疾病，由一种原生动物寄生虫引起，分布于五大洲的98个以上国家。内脏利什曼病是该疾病的主要形式之一，主要由 引起，其主要传播媒介是双翅目 。2010年首次在乌拉圭记录到该传播媒介的存在，2015年在该国北部地区发生了犬内脏利什曼病的本土疫情。我们报告了在无血清、添加胎牛血清的限定培养基中分离出导致上述疫情的5株菌株，并对它们作为前鞭毛体的生长、在人源单核细胞中的感染性和复制以及耐药性进行了表征。结果表明，这些菌株之间前鞭毛体生长相似，与来自南美的参考菌株相比，从乌拉圭疫情中分离出的5株菌株的感染性和复制能力增强，米替福新和硝呋替莫的药敏性相当，乌拉圭菌株两性霉素B的IC值与参考菌株有显著差异，高出3 - 4倍。
