College College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, PR China.
College of Environmental Science and Engineering, Ocean University of China, Qingdao, China.
Crit Rev Food Sci Nutr. 2022;62(16):4309-4325. doi: 10.1080/10408398.2021.1873734. Epub 2021 Jan 22.
There is an emergent demand for sustainable and alternative protein sources such as insects and microorganisms that meet the nutritional requirements. Microalgae possess valuable substances that could satisfy the population's dietary requirement, medicinal purpose, and energy, aligned with effective processing techniques. Several disruption techniques were applied to microalgae species for protein recovery and other compounds. The thick microalgae cell wall makes it difficult to recover all the valuable biomolecules through several downstream processes. Thus, forethought key factors need to be considered when choosing a cell lysis method. The most challenging and crucial issue is selecting a technique that requires consideration of their ability to disrupt all cell types, easy to use, purity degree, reproducible, scalable, and energy efficient. This review aims to provide useful information specifically on mechanical and non-mechanical disruption methods, the status and potential in protein extraction capacities, and constraints. Therefore, further attention in the future on potential technologies, namely explosive decompression, microfluidization, pulsed arc technology, is required to supplement the discussed techniques. This article summarizes recent advances in cell disruption methods and demonstrates insights on new directions of the techniques and future developments.
人们对可持续且可替代的蛋白质来源（如满足营养需求的昆虫和微生物）的需求日益增长。微藻拥有有价值的物质，可以满足人口的饮食需求、药用目的和能源需求，并结合有效的加工技术。已经应用了几种破坏技术来从微藻物种中回收蛋白质和其他化合物。厚厚的微藻细胞壁使得通过几种下游工艺难以回收所有有价值的生物分子。因此，在选择细胞裂解方法时需要考虑一些关键因素。最具挑战性和关键的问题是选择一种能够破坏所有细胞类型的技术，这种技术需要易于使用、纯度高、可重复、可扩展且节能。本综述旨在提供有关机械和非机械破坏方法的有用信息，特别介绍了在蛋白质提取能力方面的现状和潜力，以及存在的限制。因此，未来需要进一步关注潜在技术，例如爆炸减压、微流化、脉冲电弧技术，以补充所讨论的技术。本文总结了细胞破碎方法的最新进展，并展示了对技术新方向和未来发展的见解。
