Motoyama T, Watanabe T, Okazaki E, Tanaka N, Watanabe H
Department of Pathology, Niigata University School of Medicine, Japan.
Acta Cytol. 1995 Mar-Apr;39(2):164-70.
Although there is general agreement that immunohistochemical methods can aid in the pathologic diagnosis of malignant mesothelioma, some studies have produced conflicting results. To obtain comparable and reproducible results, unequivocal malignant mesotheliomas were studied with the biotin-streptavidin-peroxidase complex method in 14 formalin-fixed, paraffin-embedded tissue specimens, 5 ethanol-fixed smear slides and 3 cold acetone-fixed smear slides. The expression of CA-125, epithelial membrane antigen (EMA) and vimentin by malignant epithelial mesothelioma cells was hindered by their poor preservation in formalin fixative. Cytologic specimens fixed in cold acetone were the best type for immunohistochemistry. The majority of malignant epithelial mesothelioma cells in the smear slides fixed in cold acetone were positive for CA-125, EMA, low-molecular-weight cytokeratin and vimentin, but none of them were positive for carcinoembryonic antigen, CA-19-9, epithelial antigen, high-molecular-weight cytokeratin or Leu-M1.
尽管人们普遍认为免疫组织化学方法有助于恶性间皮瘤的病理诊断，但一些研究得出了相互矛盾的结果。为了获得可比且可重复的结果，采用生物素-链霉亲和素-过氧化物酶复合物法对14份福尔马林固定、石蜡包埋的组织标本、5份乙醇固定涂片玻片和3份冷丙酮固定涂片玻片进行了明确的恶性间皮瘤研究。恶性上皮性间皮瘤细胞中CA-125、上皮膜抗原（EMA）和波形蛋白的表达因在福尔马林固定剂中保存不佳而受到阻碍。冷丙酮固定的细胞学标本是免疫组织化学的最佳类型。冷丙酮固定涂片玻片中的大多数恶性上皮性间皮瘤细胞对CA-125、EMA、低分子量细胞角蛋白和波形蛋白呈阳性，但对癌胚抗原、CA-19-9、上皮抗原、高分子量细胞角蛋白或Leu-M1均无阳性反应。
