Department of Haematology and Transfusion, Royal North Shore Hospital, St Leonards, Australia.
Northern Blood Research Centre, Kolling Institute of Medical Research, St Leonards, Australia.
Br J Haematol. 2021 May;193(4):841-844. doi: 10.1111/bjh.17316. Epub 2021 Jan 22.
Clonal haematopoiesis of indeterminant potential (CHIP) increases in frequency with age. The effect of CHIP on the mobilization of autologous CD34+ peripheral blood stem cells (PBSC) has not been reported. This study uses a DNA-based targeted candidate gene approach to identify the presence of somatic mutations in ASXL1, DNMT3A, JAK2, SF3B1, TET2 and TP53 in CD34+ haematopoietic progenitor cell-apheresis products of 96 patients who undergo PBSC mobilization for autologous stem cell transplantation (ASCT). Variants were identified in a significantly greater proportion of patients who experience poor CD34+ PBSC mobilization. A DNA-based targeted candidate gene array is able to predict poor CD34+ PBSC mobilization and may be deployed pre-emptively to minimize mobilization and graft failures.
不确定潜能的克隆性造血（CHIP）随年龄增长而增加。CHIP 对自体 CD34+ 外周血造血干细胞（PBSC）动员的影响尚未报道。本研究采用基于 DNA 的靶向候选基因方法，在接受自体干细胞移植（ASCT）的 96 例患者的 CD34+ 造血祖细胞-细胞分离产品中，鉴定 ASXL1、DNMT3A、JAK2、SF3B1、TET2 和 TP53 中的体细胞突变的存在。在经历 CD34+ PBSC 动员不良的患者中，明显有更大比例的患者存在变异。基于 DNA 的靶向候选基因阵列能够预测 CD34+ PBSC 动员不良，并且可以预先部署以最小化动员和移植物失败。
