Center for Electronic, Optoelectronic and Telecommunications (CEOT), University of Algarve, Faro, Portugal.
Escola Superior de Educação e Comunicação (ESEC), University of Algarve, Faro, Portugal.
PLoS One. 2021 Jan 22;16(1):e0245148. doi: 10.1371/journal.pone.0245148. eCollection 2021.
The pathological interaction between oak trees and Phytophthora cinnamomi has implications in the cork oak decline observed over the last decades in the Iberian Peninsula. During host colonization, the phytopathogen secretes effector molecules like elicitins to increase disease effectiveness. The objective of this study was to unravel the proteome changes associated with the cork oak immune response triggered by P. cinnamomi inoculation in a long-term assay, through SWATH-MS quantitative proteomics performed in the oak leaves. Using the Arabidopis proteome database as a reference, 424 proteins were confidently quantified in cork oak leaves, of which 80 proteins showed a p-value below 0.05 or a fold-change greater than 2 or less than 0.5 in their levels between inoculated and control samples being considered as altered. The inoculation of cork oak roots with P. cinnamomi increased the levels of proteins associated with protein-DNA complex assembly, lipid oxidation, response to endoplasmic reticulum stress, and pyridine-containing compound metabolic process in the leaves. In opposition, several proteins associated with cellular metabolic compound salvage and monosaccharide catabolic process had significantly decreased abundances. The most significant abundance variations were observed for the Ribulose 1,5-Bisphosphate Carboxylase small subunit (RBCS1A), Heat Shock protein 90-1 (Hsp90-1), Lipoxygenase 2 (LOX2) and Histone superfamily protein H3.3 (A8MRLO/At4G40030) revealing a pertinent role for these proteins in the host-pathogen interaction mechanism. This work represents the first SWATH-MS analysis performed in cork oak plants inoculated with P. cinnamomi and highlights host proteins that have a relevant action in the homeostatic states that emerge from the interaction between the oomycete and the host in the long term and in a distal organ.
橡树与嗜胴菌的病理相互作用对过去几十年来伊比利亚半岛观察到的栓皮栎衰退有影响。在宿主定殖过程中，植物病原体分泌效应分子，如激发素，以提高疾病的有效性。本研究的目的是通过在长期试验中用嗜胴菌接种栓皮栎来揭示与栓皮栎免疫反应相关的蛋白质组变化，使用 SWATH-MS 定量蛋白质组学在栎树叶中进行。使用拟南芥蛋白质组数据库作为参考，在栓皮栎叶片中可自信地定量 424 种蛋白质，其中 80 种蛋白质的水平在接种和对照样品之间的 p 值低于 0.05 或变化倍数大于 2 或小于 0.5，被认为发生了改变。将嗜胴菌接种到栓皮栎根部增加了与蛋白质-DNA 复合物组装、脂质氧化、内质网应激反应以及含吡啶化合物代谢过程相关的蛋白质水平。相反，几种与细胞代谢化合物挽救和单糖分解代谢过程相关的蛋白质的丰度显著降低。观察到的最显著的丰度变化发生在核酮糖 1,5-二磷酸羧化酶小亚基（RBCS1A）、热休克蛋白 90-1（Hsp90-1）、脂氧合酶 2（LOX2）和组蛋白超家族蛋白 H3.3（A8MRLO/At4G40030），这表明这些蛋白质在宿主-病原体相互作用机制中具有重要作用。这项工作代表了首次在接种嗜胴菌的栓皮栎植物中进行的 SWATH-MS 分析，并强调了在长期和远距离器官中，宿主蛋白在由菌和宿主相互作用产生的稳态中具有重要作用。
