Pinto Patricia I S, Andrade André R, Thorne Michael A S, Estêvão M Dulce, Canario Adelino V M, Power Deborah M
Centro de Ciencias do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, Edifício 7, 8005-139, Faro, Portugal.
British Antarctic Survey (BAS), High Cross, Madingley Road, Cambridge, CB3 0ET, UK.
Data Brief. 2019 Oct 15;27:104587. doi: 10.1016/j.dib.2019.104587. eCollection 2019 Dec.
Fish scales are mineralized structures that play important roles in protection and mineral homeostasis. This tissue expresses multiple estrogen receptor subtypes and can be targeted by estrogens or estrogenic endocrine-disrupting compounds, but their effects are poorly explored. The transcriptome data here presented support the findings reported in the research article "" [1]. Juvenile sea bass were exposed to estradiol and the phytoestrogen genistein for 1 and 5 days, by intraperitoneal injections, and the effects on scale transcript expression were analysed by RNA-seq using an Illumina Hi-seq 1500. The raw reads of the 30 libraries produced have been deposited in the NCBI-SRA database with the project accession number SRP102504. Mapping of RNA-seq reads against the sea bass reference genome using the Cufflinks/TopHat package identified 371 genes that had significant (FDR<0.05) differential expression with the estradiol or genistein treatments in relation to the control scales at each exposure time, 254 of which presented more than a 2-fold change in expression. The identity of the differentially expressed genes was obtained using both automatic and manual annotations against multiple public sequence databases and they were grouped according to their patterns of expression using hierarchical clustering and heat-maps. The biological processes and KEGG pathways most significantly affected by the estradiol and/or genistein treatments were identified using Cytoscape/ClueGO enrichment analyses.
鱼鳞是矿化结构，在保护和矿物质稳态中发挥重要作用。该组织表达多种雌激素受体亚型，可被雌激素或具有雌激素活性的内分泌干扰化合物作用，但对其影响的研究较少。本文呈现的转录组数据支持了研究文章“[1]”中报道的研究结果。通过腹腔注射，将幼年海鲈暴露于雌二醇和植物雌激素染料木黄酮中1天和5天，并使用Illumina Hi-seq 1500通过RNA测序分析对鳞片转录表达的影响。所产生的30个文库的原始读数已存入NCBI-SRA数据库，项目登录号为SRP102504。使用Cufflinks/TopHat软件包将RNA测序读数与海鲈参考基因组进行比对，确定了371个基因在每个暴露时间与对照鳞片相比，在雌二醇或染料木黄酮处理下具有显著（FDR<0.05）差异表达，其中254个基因的表达变化超过2倍。使用针对多个公共序列数据库的自动和手动注释获得差异表达基因的身份，并使用层次聚类和热图根据其表达模式进行分组。使用Cytoscape/ClueGO富集分析确定受雌二醇和/或染料木黄酮处理影响最显著的生物学过程和KEGG途径。
