Balotf Sadegh, Wilson Calum R, Tegg Robert S, Nichols David S, Wilson Richard
New Town Research Laboratories, Tasmanian Institute of Agriculture, University of Tasmania, New Town, TAS, Australia.
Central Science Laboratory, University of Tasmania, Hobart, TAS, Australia.
Front Plant Sci. 2022 Apr 14;13:872901. doi: 10.3389/fpls.2022.872901. eCollection 2022.
Potato is one of the most important food crops for human consumption. The soilborne pathogen infects potato roots and tubers, resulting in considerable economic losses from diminished tuber yields and quality. A comprehensive understanding of how potato plants respond to infection is essential for the development of pathogen-resistant crops. Here, we employed label-free proteomics and phosphoproteomics to quantify systemically expressed protein-level responses to root infection in potato foliage of the susceptible and resistant potato cultivars. A total of 2,669 proteins and 1,498 phosphoproteins were quantified in the leaf samples of the different treatment groups. Following statistical analysis of the proteomic data, we identified oxidoreductase activity, electron transfer, and photosynthesis as significant processes that differentially changed upon root infection specifically in the resistant cultivar and not in the susceptible cultivar. The phosphoproteomics results indicated increased activity of signal transduction and defense response functions in the resistant cultivar. In contrast, the majority of increased phosphoproteins in the susceptible cultivar were related to transporter activity and sub-cellular localization. This study provides new insight into the molecular mechanisms and systemic signals involved in potato resistance to infection and has identified new roles for protein phosphorylation in the regulation of potato immune response.
马铃薯是人类食用的最重要粮食作物之一。这种土传病原菌会感染马铃薯的根和块茎，导致块茎产量和品质下降，造成相当大的经济损失。全面了解马铃薯植株对该病原菌感染的反应，对于培育抗病作物至关重要。在此，我们采用无标记蛋白质组学和磷酸化蛋白质组学方法，对感病和抗病马铃薯品种叶片中因根部感染而系统表达的蛋白质水平反应进行定量分析。在不同处理组的叶片样本中，共定量分析了2669种蛋白质和1498种磷酸化蛋白质。对蛋白质组数据进行统计分析后，我们确定氧化还原酶活性、电子传递和光合作用是在根部感染后有显著差异变化的重要过程，且这种变化在抗病品种中尤为明显，而在感病品种中则不然。磷酸化蛋白质组学结果表明，抗病品种中信号转导和防御反应功能的活性增强。相比之下，感病品种中大多数磷酸化蛋白质的增加与转运蛋白活性和亚细胞定位有关。本研究为马铃薯对该病原菌感染的抗性所涉及的分子机制和系统信号提供了新的见解，并确定了蛋白质磷酸化在调节马铃薯免疫反应中的新作用。
