Department of Spine Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Department of Orthopedic, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
Eur J Pharmacol. 2021 Mar 15;895:173891. doi: 10.1016/j.ejphar.2021.173891. Epub 2021 Jan 19.
Intervertebral disc degeneration (IDD) is a spinal degenerative disease and one of the most important causes of musculoskeletal disability. Matrix metalloproteinase (MMP)-mediated extracellular matrix degradation is the core process of IDD. The regulators of MMPs in the intervertebral disc are still not fully known. In this study, using quantitative reverse transcription PCR, luciferase reporter assay, Western blotting, immunofluorescence, flow cytometry, and Cell Counting Kit-8 assay, we found that the miR-874-3p expression level was significantly decreased in IDD patients. MiR-874-3p could target and repress MMP2 and MMP3 expression in nucleus pulposus cells. These results could improve the understanding of IDD and provide a possible diagnostic marker and treatment candidate for IDD. The miR-874-3p/MMP2/MMP3 axis might also provide direction for future cancer and inflammation investigations.
椎间盘退变性疾病（IDD）是一种脊柱退行性疾病，也是肌肉骨骼功能障碍的最重要原因之一。基质金属蛋白酶（MMP）介导的细胞外基质降解是 IDD 的核心过程。椎间盘 MMP 的调节剂尚不完全清楚。在这项研究中，我们通过定量逆转录 PCR、荧光素酶报告基因检测、Western blot、免疫荧光、流式细胞术和细胞计数试剂盒-8 检测发现，IDD 患者的 miR-874-3p 表达水平显著降低。miR-874-3p 可以靶向并抑制髓核细胞中 MMP2 和 MMP3 的表达。这些结果可以加深对 IDD 的认识，并为 IDD 提供一个可能的诊断标志物和治疗候选物。miR-874-3p/MMP2/MMP3 轴也可能为未来的癌症和炎症研究提供方向。
