Nissi Ritva, Santala Markku, Talvensaari-Mattila Anne
Department of Obstetrics and Gynecology, Oulu University Hospital, PO Box 5000, 90014, Oulu, Finland.
BMC Res Notes. 2021 Jan 22;14(1):31. doi: 10.1186/s13104-021-05442-w.
Matrix metalloproteinases (MMPs) are important regulators of vascular and uterine remodeling. They exhibit proteolytic activity implicating the efficiency of trophoblast invasion to the uterine wall involving marked hemodynamic and uterine changes. In this pilot study sera of 13 women with normal pregnancy was analyzed to evaluate the usage of MMPs as diagnostic tool. The concentrations of circulating MMP-9, MMP-2/TIMP-2 complex and TIMP-1 in different time points during normal pregnancy has not been studied. The serum levels of MMP-9, TIMP-1, TIMP-2 and MMP-2/TIMP-2 complex were determined by enzyme-linked immunosorbent assay (ELISA). Using the same method, we have shown that serum MMPs are elevated in spontaneous early pregnancy failure as compared to normal pregnancy.
The serum levels of MMP-9 and TIMP-1 were stable throughout pregnancy. The level of MMP-2/TIMP-2 complex was slightly increased after week 15 without statistical significance. For our best knowledge, this is a first study of the serum levels of MMP-9, MMP-2/TIMP-2 and TIMP-1 on different time points during normal pregnancy. Further measurements with the correlation to the outcome of the pregnancy are needed.
基质金属蛋白酶（MMPs）是血管和子宫重塑的重要调节因子。它们表现出蛋白水解活性，这与滋养层侵入子宫壁的效率有关，涉及显著的血流动力学和子宫变化。在这项初步研究中，分析了13名正常妊娠女性的血清，以评估MMPs作为诊断工具的用途。正常妊娠不同时间点循环MMP-9、MMP-2/TIMP-2复合物和TIMP-1的浓度尚未得到研究。通过酶联免疫吸附测定（ELISA）测定血清中MMP-9、TIMP-1、TIMP-2和MMP-2/TIMP-2复合物的水平。使用相同的方法，我们已经表明，与正常妊娠相比，血清MMPs在自然早期妊娠失败中升高。
整个孕期MMP-9和TIMP-1的血清水平稳定。MMP-2/TIMP-2复合物水平在第15周后略有升高，但无统计学意义。据我们所知，这是首次对正常妊娠不同时间点的血清MMP-9、MMP-2/TIMP-2和TIMP-1水平进行研究。需要进一步测量并与妊娠结局相关联。
