Harkema J R, Hotchkiss J A, Harmsen A G, Henderson R F
Lovelace Inhalation Toxicology Research Institute, Albuquerque, NM 87185.
Am J Pathol. 1988 Mar;130(3):605-15.
Certain inhaled toxicants are known to induce mucous hypersecretion in the respiratory epithelium. This secretory change may be a direct effect of the toxicant or an indirect effect of the concomitant inflammatory response. The present study was designed to determine by quantitative histochemistry whether the influx of neutrophils through the nasal respiratory epithelium would induce significant quantitative changes in the amount of intraepithelially stored mucosubstance. F344/N rats were intranasally instilled with endotoxin to elicit a transient influx of neutrophils into the nasal epithelium. Peak intraepithelial infiltration of neutrophils was evident 6 hours after instillation. There was a concurrent quantitative decrease in stored epithelial mucosubstance at the same time after instillation. Amounts of epithelial mucosubstance returned to that measured prior to neutrophil infiltration by 24 hours after instillation, when intraepithelial neutrophils were diminishing. Rats in which circulating neutrophils were sequestered in the lungs and prevented from migrating into endotoxin-exposed nasal epithelium had no change in the quantity of stored mucosubstance 6 hours after instillation. Therefore, it is concluded that a transepithelial migration of neutrophils elicits a transient depletion of stored mucosubstances in the nasal respiratory epithelium. Whether this is due to the release of secretagogues from the migrating leukocytes or another neutrophil-related method of stimulating mucous secretion is not known.
已知某些吸入性毒物会诱导呼吸道上皮的黏液分泌亢进。这种分泌变化可能是毒物的直接作用，也可能是伴随的炎症反应的间接作用。本研究旨在通过定量组织化学确定中性粒细胞通过鼻呼吸上皮的流入是否会导致上皮内储存的黏液物质数量发生显著的定量变化。将内毒素经鼻滴注到F344/N大鼠体内，以引发中性粒细胞短暂流入鼻上皮。滴注后6小时，中性粒细胞的上皮内浸润达到峰值。滴注后同时，储存的上皮黏液物质出现定量减少。当上皮内中性粒细胞减少时，滴注后24小时上皮黏液物质的量恢复到中性粒细胞浸润前测得的水平。将循环中的中性粒细胞隔离在肺部并阻止其迁移到暴露于内毒素的鼻上皮中的大鼠，滴注后6小时储存的黏液物质数量没有变化。因此，得出结论，中性粒细胞的跨上皮迁移会引起鼻呼吸上皮中储存的黏液物质短暂耗竭。这是否是由于迁移的白细胞释放促分泌素或另一种与中性粒细胞相关的刺激黏液分泌的方法尚不清楚。
