Hochstrasser K, Albrecht G J, Schönberger O L, Rasche B, Lempart K
Hoppe Seylers Z Physiol Chem. 1981 Oct;362(10):1369-75. doi: 10.1515/bchm2.1981.362.2.1369.
It was shown previously that human bronchial mucus contains an acid-stable proteinase inhibitor directed against trypsin and chymotrypsin, polymorphonuclear granulocyte elastase and cathepsin G. In addition to this well-characterized inhibitor, designated here as BSI-ATE (identical with the inhibitor HUSI-I from human seminal plasma or antileucoprotease), another acid-stable inhibitor BSI-E is present in the mucus which exerts inhibitory activity towards porcine pancreatic and human granulocytic elastase, but not against trypsin, chymotrypsin, or granulocytic cathepsin G. This elastase-specific inhibitor was isolated by affinity chromatography. Its molecular mass and its amino acid composition are very similar to those of BSI-TE. An immunological cross-reactivity between both inhibitor species was not observed. In the mucus of patients suffering from obstructive airway disease the elastase-specific inhibitor is not present in the free form but can be liberated by acidification.
先前的研究表明，人支气管黏液中含有一种对胰蛋白酶、糜蛋白酶、多形核粒细胞弹性蛋白酶和组织蛋白酶G具有酸稳定性的蛋白酶抑制剂。除了这种特性明确的抑制剂（在此称为BSI-ATE，与人精浆中的抑制剂HUSI-I或抗白细胞蛋白酶相同）之外，黏液中还存在另一种酸稳定抑制剂BSI-E，它对猪胰弹性蛋白酶和人粒细胞弹性蛋白酶具有抑制活性，但对胰蛋白酶、糜蛋白酶或粒细胞组织蛋白酶G没有抑制作用。这种弹性蛋白酶特异性抑制剂通过亲和层析法分离得到。其分子量和氨基酸组成与BSI-TE非常相似。未观察到这两种抑制剂之间的免疫交叉反应。在患有阻塞性气道疾病的患者的黏液中，弹性蛋白酶特异性抑制剂不是以游离形式存在，而是可以通过酸化释放出来。
