Cao Jianhua, Lu Zijuan, Qin Xuemei, Li Zhenyu
Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China.
J Mass Spectrom. 2021 Feb;56(2):e4697. doi: 10.1002/jms.4697.
Farfarae Flos (FF) has been used in China for a long time as an anti-tussive and expectorant herbal drugs, and it was usually honey-fried FF (HFF). To clarify the mechanism of honey processing, it is important to know the chemical difference between FF and HFF firstly. In this study, UHPLC-Orbitrap-MS was used to characterize the chemical compounds in FF, honey and HFF. Then the metabolomic approach based on UHPLC-Orbitrap-MS revealed 68 differential compounds between the FF and HFF, and chemical reactions occurring during processing were also proposed to elucidate the honey processing mechanisms of FF. In order to investigate the chemical difference between FF and HFF comprehensively and accurately, the components derived from the honey and the moisture content in FF and HFF were considered for the first time. In summary, this study investigated the chemical differences between FF and HFF in a holistic way, which laid the basis for the quality control of HFF and further explaining the honey processing mechanisms of FF. In addition, eight native compounds derived from the honey could be used as the index to authenticate the HFF prepared by the genuine honey.
款冬花在中国长期以来一直被用作止咳祛痰的草药，通常为蜜炙款冬花。为阐明蜜制的机制，首先了解款冬花与蜜炙款冬花之间的化学差异很重要。在本研究中，采用超高效液相色谱-轨道阱质谱法对款冬花、蜂蜜和蜜炙款冬花中的化学成分进行表征。然后基于超高效液相色谱-轨道阱质谱法的代谢组学方法揭示了款冬花与蜜炙款冬花之间的68种差异化合物，并提出了加工过程中发生的化学反应以阐明款冬花的蜜制机制。为全面、准确地研究款冬花与蜜炙款冬花之间的化学差异，首次考虑了蜂蜜衍生的成分以及款冬花和蜜炙款冬花中的水分含量。总之，本研究以整体的方式研究了款冬花与蜜炙款冬花之间的化学差异，为蜜炙款冬花的质量控制和进一步解释款冬花的蜜制机制奠定了基础。此外，八种源自蜂蜜的天然化合物可作为鉴定由纯正蜂蜜制备的蜜炙款冬花的指标。
