Cao Juan, Wang Fu-Gang, Liu Ke, Liu Wen-Qing
School of Pharmacy, Taishan Medical University, Taian 271016, China.
Zhong Yao Cai. 2012 Jan;35(1):33-6.
To establish a HPLC-FP for the determination of honey-fried Farfarae Flos.
HPLC analysis was performed on a C18 column (4.6 mm x 250 mm, 5 microm), the mobile phase consisted of 0.1% phosphoric acid solution and acetonitrile as gradient elution with the flow rate of 1.0 mL/min. The wavelength was 240 nm.
HPLC fingerprint of honey-fried Farfarae Flos was established and the results of methodological study met the technical requirements for fingerprint.
The established method can be used for quality control of honey-fried Farfarae Flos.
建立蜜炙款冬花的高效液相色谱指纹图谱测定法。
采用C18柱（4.6 mm×250 mm，5μm）进行高效液相色谱分析，流动相为0.1%磷酸溶液和乙腈，梯度洗脱，流速为1.0 mL/min，检测波长为240 nm。
建立了蜜炙款冬花的高效液相色谱指纹图谱，方法学研究结果符合指纹图谱技术要求。
所建立的方法可用于蜜炙款冬花的质量控制。
