Institute of Taihang Materia Medica, Shanxi University of Chinese Medicine, Jinzhong 030619, China; Experimental Management Center, Shanxi University of Chinese Medicine, Jinzhong 030619, China.
Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2024 Jun 1;1240:124145. doi: 10.1016/j.jchromb.2024.124145. Epub 2024 May 4.
Farfarae Flos is a traditional herb widely employed for treating coughs, bronchitis, and asthmatic disorders. In the current study, we utilized SWATH and IDA data acquisition modes in combination with multiple data processing techniques to identify Farfarae Flos metabolites in mice serum. A total of 56 compounds were characterized, including 31 phenolic acids, 13 flavonoids, 11 sesquiterpenoids and 1 alkaloid. Further quantitative analysis was conducted on 12 absorbed metabolites, utilizing a newly developed and rigorously validated analytical method. Our approach demonstrated an acceptable level of specificity, accuracy, precision, and stability. When applied to compare the serum of mice treated with FF, all 12 metabolites showed the highest concentration at 0.5 h. Overall, this study presented a novel strategy for unraveling the active compounds of FF via serum pharmacochemistry analysis, which made a foundation for exploring the pharmacodynamic material basis of FF.
款冬花是一种传统草药，广泛用于治疗咳嗽、支气管炎和哮喘等疾病。在本研究中，我们采用 SWATH 和 IDA 数据采集模式结合多种数据处理技术，鉴定了小鼠血清中的款冬花代谢产物。共鉴定出 56 种化合物，包括 31 种酚酸、13 种黄酮类化合物、11 种倍半萜类化合物和 1 种生物碱。利用新开发的经过严格验证的分析方法，对 12 种吸收代谢物进行了定量分析。我们的方法表现出可接受的特异性、准确性、精密度和稳定性。当应用于比较经 FF 处理的小鼠血清时，所有 12 种代谢物在 0.5 h 时均显示出最高浓度。总的来说，本研究通过血清药物化学分析为揭示 FF 的活性化合物提供了一种新策略，为探索 FF 的药效物质基础奠定了基础。
