School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang, 212100, PR China; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang, 212100, PR China.
Microb Pathog. 2021 Feb;151:104749. doi: 10.1016/j.micpath.2021.104749. Epub 2021 Jan 20.
Callosobruchus chinensis is one of the important postharvest pests in legume growing areas. Bacterial pesticide is a potential alternative method to control storage pests. However, the effect of these pathogen bacteria on storage pests, and the molecular mechanisms of insect response remain to be to investigated.
Using the next generation sequencing technology, we established a transcriptomic library for C. chinensis larvae in response to Escherichia coli. Total of 355 differential expressed genes (DEGs) were identified, which 178 DEGs were upregulated, and 177 DEGs were downregulated compared to control group. To validate the RNA-seq analysis, 20 DEGs and 14 immune-related genes were selected to perform quantitative polymerase chain reaction (RT-qPCR). These immune-related genes were involved in recognition (peptidoglycan recognition proteins), signal transduction (fibrinogen-related proteins, serine proteinases and NF-κB), and execution effectors (phenoloxidase, defensin, attacin, and antimicrobial peptide). In addition, genes that encode digestive and respiratory enzymes were altered in C. chinensis larvae in response to infection. Some genes that involved in juvenile hormone and insulin pathway appeared to express differentially, suggesting that pathogen infection might lead to developmental arrest. Furthermore, iron homeostasis and chitin metabolism appeared significantly altered after infection.
In this study, we characterized the immune response of C. chinensis larvae in response to E. coli using RNA-seq, from pathogen recognition, signal transduction, to execution. Some other identified genes were involved in iron homeostasis, respiration, and digestion. A better understanding of molecular response of beetle to pathogen will facilitate us to develop an available strategy to control storage pests.
家蚕是豆类种植区重要的仓储害虫之一。细菌农药是一种有潜力的防治仓储害虫的替代方法。然而，这些病原菌对仓储害虫的作用，以及昆虫的反应的分子机制仍有待研究。
使用下一代测序技术，我们建立了家蚕幼虫对大肠杆菌反应的转录组文库。与对照组相比，共鉴定出 355 个差异表达基因（DEGs），其中 178 个上调，177 个下调。为了验证 RNA-seq 分析的结果，选择了 20 个 DEGs 和 14 个免疫相关基因进行实时定量聚合酶链反应（RT-qPCR）。这些免疫相关基因参与识别（肽聚糖识别蛋白）、信号转导（纤维蛋白原相关蛋白、丝氨酸蛋白酶和 NF-κB）和执行效应子（酚氧化酶、防御素、Attacin 和抗菌肽）。此外，家蚕幼虫中与消化和呼吸相关的酶基因也发生了改变。一些参与保幼激素和胰岛素途径的基因似乎表达不同，这表明病原菌感染可能导致发育停滞。此外，铁稳态和几丁质代谢在感染后明显改变。
在这项研究中，我们使用 RNA-seq 对家蚕幼虫对大肠杆菌的免疫反应进行了表征，从病原体识别、信号转导到执行。其他一些已鉴定的基因参与铁稳态、呼吸和消化。更好地了解甲虫对病原体的分子反应将有助于我们开发有效的仓储害虫防治策略。
