Cui S F, Wang L, Ma L, Wang Y L, Qiu J P, Liu Zh Ch, Geng X Q
School of Grain Science and Technology,Jiangsu University of Science and Technology,Zhenjiang 212004,China.
Department of Resources and Environment,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China.
Bull Entomol Res. 2019 Apr;109(2):266-277. doi: 10.1017/S0007485318000512. Epub 2018 Jul 12.
Stored product insects show high adaption to hypoxia and hypercapnia, but the underlying mechanism is still unclear. Herein, a comparative transcriptome on 4th adzuki bean weevil (Callosobruchus chinensis) instar larvae was studied to clarify the response mechanisms to hypoxia (HA) and hypoxia/hypercapnia (HHA) using NextSeq500 RNA-Seq. Transcript profiling showed a significant difference in HA or HHA exposure both quantitatively and qualitatively. Compared with control, 631 and 253 genes were significantly changed in HHA and HA, respectively. Comparing HHA with HA, 1135 differentially expressed genes (DEGs) were identified. The addition of hypercapnia made a complex alteration on the hypoxia response of bean weevil transcriptome, carbohydrate, energy, lipid and amino acid metabolism were the most highly enriched pathways for genes significantly changed. In addition, some biological processes that were not significantly enriched but important were also discussed, such as immune system and signal transduction. Most of the DEGs related to metabolism both in HHA and HA were up-regulated, while the DEGs related to the immune system, stress response or signal transduction were significantly down-regulated or suppressed. This research reveals a comparatively full-scale result in adzuki bean weevil hypoxia and hypoxia/hypercapnia tolerance mechanism at transcription level, which might provide new insights into the genomic research of this species.
仓储害虫对低氧和高二氧化碳环境具有高度适应性，但其潜在机制仍不清楚。在此，利用NextSeq500 RNA测序技术对四纹豆象（Callosobruchus chinensis）四龄幼虫进行了比较转录组学研究，以阐明其对低氧（HA）和低氧/高二氧化碳（HHA）的响应机制。转录谱分析表明，在HA或HHA处理下，转录本在数量和质量上均存在显著差异。与对照相比，HHA和HA处理分别有631个和253个基因发生显著变化。比较HHA和HA，共鉴定出1135个差异表达基因（DEG）。高二氧化碳的添加使豆象转录组的低氧反应发生了复杂变化，碳水化合物、能量、脂质和氨基酸代谢是显著变化基因中富集程度最高的途径。此外，还讨论了一些虽未显著富集但很重要的生物学过程，如免疫系统和信号转导。在HHA和HA处理中，大多数与代谢相关的DEG均上调，而与免疫系统、应激反应或信号转导相关的DEG则显著下调或受到抑制。本研究揭示了四纹豆象在转录水平上对低氧和低氧/高二氧化碳耐受性机制的较为全面的结果，这可能为该物种的基因组研究提供新的见解。
