Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, PMB 12003, Surulere, Lagos, Nigeria.
Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, PMB 12003, Surulere, Lagos, Nigeria.
J Ethnopharmacol. 2021 Jun 12;273:113870. doi: 10.1016/j.jep.2021.113870. Epub 2021 Jan 21.
The seed of Avocado (Persea americana, Lauraceae), non-edible part of the fruit is used as health product. It has been reported as traditional female contraceptive and sterilizer in Peru and some Asian countries and in Nigeria as cardio-protective agent. The present study focused on the effect of hydro-methanolic seed extract of Persea americana on female hormones and toxicity profile using animal models.
The serum follicle stimulating hormone (FSH) and progesterone (PROG) concentrations in mature non-pregnant female rats were assayed using hormonal kits. The toxicity profile was assessed using Lorke's acute toxicity model, haemato-biochemical evaluation and histopathological studies of reproductive related organs. Parameters were measured on day-30, 60 and 90. Presence of biomarker flavonoid compounds were confirmed using High Performance Liquid Chromatography.
The extract at 20, 100 and 500 mg kg altered FSH and PROG hormone profile of the treated groups. The extract initially, dose-dependently decreased FSH level in day-30 (6.95, 3.97, 2.08 IU/L respectively) compared to untreated group before a significant increase was observed for day 60 and 90. Progesterone increased dose-dependently in the treated groups throughout the 90-day treatment duration. This may be Indicating cumulative effect on the hormone. No deleterious or toxicity effect was noticed.
The extract of Persea americana seed affects female hormone activity. This may find application in various hormonal management procedures, maternal and reproductive health and fertility control/help health facilities. However, it should be used with caution in women intending to conceive.
鳄梨（Persea americana，樟科）的种子，果实的不可食用部分，被用作保健品。它在秘鲁和一些亚洲国家被报道为传统的女性避孕药和绝育剂，在尼日利亚则被用作心脏保护剂。本研究集中于研究鳄梨的水-甲醇种子提取物对女性激素的影响，并使用动物模型评估其毒性概况。
使用激素试剂盒检测成熟未怀孕雌性大鼠的血清促卵泡激素（FSH）和孕激素（PROG）浓度。使用 Lorke 的急性毒性模型、血液生化评估和生殖相关器官的组织病理学研究来评估毒性概况。在第 30、60 和 90 天测量参数。使用高效液相色谱法确认生物标志物类黄酮化合物的存在。
提取物在 20、100 和 500 mg/kg 剂量下改变了处理组的 FSH 和 PROG 激素谱。与未处理组相比，提取物在第 30 天（分别为 6.95、3.97、2.08 IU/L），最初剂量依赖性地降低了 FSH 水平，然后在第 60 和 90 天观察到显著增加。在整个 90 天的治疗期间，孕激素在处理组中呈剂量依赖性增加。这可能表明对激素有累积作用。没有观察到有害或毒性作用。
鳄梨种子的提取物会影响女性激素活性。这可能在各种激素管理程序、母婴和生殖健康以及生育控制/帮助保健设施中得到应用。然而，对于计划怀孕的女性，应谨慎使用。
