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采用 UPLC-ESI-Q-TOF-MS 脂质组学技术分析罗非鱼(Oreochromis niloticus)三个部位(肌肉、头部和内脏)的脂质特征。

Characterization of lipid profiling in three parts (muscle, head and viscera) of tilapia (Oreochromis niloticus) using lipidomics with UPLC-ESI-Q-TOF-MS.

机构信息

College of Food Science and Engineering, Hainan University, Haikou 570228, China; Hainan Provincial Engineering Research Centre of Aquatic Resources Efficient Utilization in the South China Sea, Haikou 570228, China; Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Haikou 570228, China.

College of Food Science and Engineering, Hainan University, Haikou 570228, China; Hainan Provincial Engineering Research Centre of Aquatic Resources Efficient Utilization in the South China Sea, Haikou 570228, China; Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Haikou 570228, China.

出版信息

Food Chem. 2021 Jun 15;347:129057. doi: 10.1016/j.foodchem.2021.129057. Epub 2021 Jan 11.

DOI:10.1016/j.foodchem.2021.129057
PMID:33484957
Abstract

A lipidomic evaluation was performed on the tilapia muscle, head and viscera, including studying the composition, distribution and stereospecific characteristics of fatty acids and lipid species. The head and viscera lipids were significantly richer than the muscle lipids. Triacylglycerols were the predominant fraction (over 80% of total lipid in the muscle and head). Additionally, polyunsaturated fatty acids had higher percentages in phospholipids (30.35-52.05% of total fatty acids) than in triacylglycerols (18.11-25.15%). The C52:2 and C52:3 were the most abundant triacylglycerols, which indicates the potential application in infant food. Moreover, 622, 530 and 513 lipids were identified using ultraperformance liquid chromatography-quadrupole time-of-flight-mass spectrometry in the muscle, head and viscera, respectively. The three tilapia parts were distinguished using multivariate analysis. Five fatty acids and 33 lipid species were considered as the potential biomarkers. This comprehensive analysis will help to evaluate the lipid nutritional values and facilitate exploitation in tilapia consumption and processing.

摘要

对罗非鱼肌肉、头部和内脏进行了脂质组学评估,包括研究脂肪酸和脂质种类的组成、分布和立体特异性。头部和内脏的脂质明显比肌肉的脂质丰富。三酰基甘油是主要成分(占肌肉和头部总脂质的 80%以上)。此外,多不饱和脂肪酸在磷脂中的百分比(总脂肪酸的 30.35-52.05%)高于三酰基甘油(18.11-25.15%)。C52:2 和 C52:3 是最丰富的三酰基甘油,这表明它们在婴儿食品中有潜在的应用。此外,使用超高效液相色谱-四极杆飞行时间质谱在肌肉、头部和内脏中分别鉴定了 622、530 和 513 种脂质。使用多元分析区分了这三个罗非鱼部分。有 5 种脂肪酸和 33 种脂质被认为是潜在的生物标志物。这项综合分析将有助于评估脂质的营养价值,并促进罗非鱼消费和加工的开发。

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