Department of Obstetrics and Gynecology, University Hospital "Sestre Milosrdnice", Zagreb, Croatia.
Department of Gynecology and Obstetrics, School of Medicine, University of Zagreb, Zagreb, Croatia; Clinic of Obstetrics and Gynecology, Clinical Hospital "Sveti Duh", Zagreb, Croatia; Centre of Excellence in Reproductive and Regenerative Medicine, University of Zagreb School of Medicine, Zagreb, Croatia.
Bosn J Basic Med Sci. 2021 Aug 1;21(4):447-453. doi: 10.17305/bjbms.2020.5422.
Dishevelled family proteins (DVL1, DVL2, and DVL3) are cytoplasmic proteins that are involved in canonical and non-canonical Wnt signaling pathway during embryonic development. The role of DVL proteins in the placental tissue remains mostly unknown. In the current study, we explored the role of Dishevelled proteins in naturally invasive tissue, trophoblast. Formalin-fixed paraffin-embedded samples of 15 term placentas from physiologic term pregnancies and 15 term placentas from pregnancies complicated with intrauterine growth restrictions (IUGR) were used for the study. Expression levels of mRNA for DVL1, DVL2, and DVL3 in placentas were analyzed by quantitative real-time PCR (qRTPCR). DVL1, DVL2, and DVL3 protein expression were semi-quantitatively analyzed using immunohistochemistry. The expression of DVL2 and DVL3 proteins was significantly higher in trophoblasts in placental villi from IUGR pregnancies compared with the control group of term placentas. In contrast, DVL3 protein expression was significantly higher in endothelial cells in placental villi from IUGR pregnancies compared with normal term placentas. The observed differences at protein levels between normal and IUGR placentas were not confirmed at the mRNA levels of DVL genes. Our data indicate the active involvement of DVL proteins in IUGR-related placentas. No significant changes were observed in DVL mRNA levels between the two groups of placentas. Further studies are required to explore the clinical relevance of these observations.
盘基蛋白家族蛋白（DVL1、DVL2 和 DVL3）是参与胚胎发育过程中经典和非经典 Wnt 信号通路的细胞质蛋白。DVL 蛋白在胎盘组织中的作用在很大程度上尚不清楚。在本研究中，我们探索了 DVL 蛋白在自然侵袭组织——滋养层中的作用。使用 15 例来自生理足月妊娠的福尔马林固定石蜡包埋的足月胎盘样本和 15 例来自伴有宫内生长受限（IUGR）的足月妊娠的胎盘样本进行研究。通过实时定量 PCR（qRT-PCR）分析胎盘中 DVL1、DVL2 和 DVL3 的 mRNA 表达水平。通过免疫组织化学半定量分析 DVL1、DVL2 和 DVL3 蛋白的表达。与对照组足月胎盘相比，IUGR 妊娠胎盘绒毛滋养层中 DVL2 和 DVL3 蛋白的表达明显更高。相反，与正常足月胎盘相比，IUGR 妊娠胎盘绒毛中内皮细胞的 DVL3 蛋白表达明显更高。在正常和 IUGR 胎盘之间在蛋白水平上观察到的差异在 DVL 基因的 mRNA 水平上没有得到证实。我们的数据表明 DVL 蛋白在与 IUGR 相关的胎盘中的积极参与。两组胎盘之间的 DVL mRNA 水平没有观察到明显变化。需要进一步研究来探讨这些观察结果的临床相关性。
