Chair of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, Poland.
Chair of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, Poland.
Anal Biochem. 2021 Mar 15;617:114117. doi: 10.1016/j.ab.2021.114117. Epub 2021 Jan 21.
The study aimed to use Natural Deep Eutectic Solvents (NADES) as an extractant of metal oxide NPs from plant material. The plant chosen for the study was radish after exposure, growing on media containing: copper(II) oxide, cerium(IV) oxide, and titanium(IV) oxide. The first step of the study was to investigate the influence of NADES on NPs. In the second step, selected NADES solvents were used as extractants of NPs present in radish after exposure. Single-particle Inductively Coupled Plasma Mass Spectrometry technique (SP-ICP-MS) was used to determine the number and size of NPs. As a result of the research, it was found that copper(II) oxide NPs, regardless of the solvent used, is not present in the extract. Only the ionic form of the element was present in the solution. Higher sized cerium(IV) oxide NPs were accumulated in the root, while smaller sized ones were found in radish leaves. The titanium(IV) oxide NPs were agglomerated and were present in a small amount in radish leaves, accumulating mainly in the root. Finally, it can be concluded that NADES allows the extraction of metal oxide NPs from the plant material.
本研究旨在利用自然深共晶溶剂（NADES）作为从植物材料中提取金属氧化物纳米颗粒（NPs）的萃取剂。研究中选择的植物是萝卜，暴露在含有氧化铜（II）、氧化铈（IV）和氧化钛（IV）的培养基上后进行生长。研究的第一步是研究 NADES 对 NPs 的影响。在第二步中，选择 NADES 溶剂作为暴露后萝卜中存在的 NPs 的萃取剂。采用单颗粒电感耦合等离子体质谱技术（SP-ICP-MS）测定 NPs 的数量和尺寸。研究结果表明，无论使用哪种溶剂，氧化铜（II） NPs 均不存在于提取物中。溶液中仅存在元素的离子形式。较大尺寸的氧化铈（IV） NPs 积聚在根部，而较小尺寸的 NPs 则存在于萝卜叶片中。氧化钛（IV） NPs 团聚，在萝卜叶片中含量较少，主要积聚在根部。最后，可以得出结论，NADES 允许从植物材料中提取金属氧化物 NPs。
