Department of Botany and Microbiology, Faculty of Science, Alexandria University, Egypt.
Marine Biotechnology and Natural Products Extract Laboratory, National Institute of Oceanography and Fisheries, Alexandria, Egypt.
J Biotechnol. 2021 Feb 20;328:95-105. doi: 10.1016/j.jbiotec.2021.01.008. Epub 2021 Jan 22.
Algae are omnipresent in all seas and oceans, which make thema target for many applications such as bio-fertilizers, fish feeding and removal of heavy metals. In the present study, different algal species were examined as sustainable alternatives substrates for PHA production by Halomonas sp. Several media simulations were utilized to achieve high polymer productivity. The maximum poly(3-hydroxybutyrate) (PHB) concentrations were determined by using Corallina mediterranea hydrolysates as a carbon and nitrogen source. The isolates Halomonas pacifica ASL10 and Halomonas salifodiane ASL11 were found to be able to produce PHA by 67 % wt and 63 % wt CDW, respectively. PHB nanoparticles (NPs) had high zeta potential values and small particle sizes. These properties make it suitable for several drug delivery and pharmaceutical applications. Interestingly, NPs showed a potent antibacterial activity against several reference strains. The antibacterial efficacy of PHA-NPs has not been previously studied, thus this study opens a promising use of PHA-NPs.
藻类在所有海洋中无处不在，这使得它们成为许多应用的目标，如生物肥料、鱼类饲料和重金属去除。在本研究中，研究了不同的藻类物种作为聚羟基脂肪酸酯（PHA）生产的可持续替代基质，通过盐单胞菌属（Halomonas）。利用几种介质模拟来实现高聚合物产率。通过使用地中海珊瑚藻水解物作为碳源和氮源，确定了最大聚（3-羟基丁酸酯）（PHB）浓度。分离株盐单胞菌属（Halomonas）pacifica ASL10 和盐单胞菌属（Halomonas）salifodiane ASL11 被发现能够分别以 67%wt 和 63%wt CDW 的产量生产 PHA。PHB 纳米颗粒（NPs）具有高的zeta 电位值和小的粒径。这些特性使其适用于多种药物输送和制药应用。有趣的是，NPs 对几种参考菌株表现出很强的抗菌活性。PHA-NPs 的抗菌功效以前没有研究过，因此本研究为 PHA-NPs 的应用开辟了广阔的前景。
