基于果糖的 Halomonas sp. YLGW01 聚 3-羟基丁酸的超量生产及碳源对细菌形态的影响。
Fructose based hyper production of poly-3-hydroxybutyrate from Halomonas sp. YLGW01 and impact of carbon sources on bacteria morphologies.
机构信息
Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, South Korea.
Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, South Korea; Institute for Ubiquitous Information Technology and Applications, Konkuk University, Seoul 05029, South Korea.
出版信息
Int J Biol Macromol. 2020 Jul 1;154:929-936. doi: 10.1016/j.ijbiomac.2020.03.129. Epub 2020 Mar 18.
Polyhydroxyalkanoates (PHA), such as poly (3-hydroxybutyrate) (PHB), have emerged as potential alternatives to petroleum-based plastics and can be produced through the appropriate selection of marine bacteria that are already adapted to high salt and low temperature conditions without the requirement of antibiotic treatment. The present study, thus, aimed to screen and characterize thirteen PHA-producing microbial strains isolated from the Gwangalli beach in Busan, South Korea. Among them, Halomonas sp. YLGW01 produced the highest amount of PHB (94.6 ± 1.8% (w/w)) using fructose. Interestingly Halomonas sp. YLGW01 showed increase in cell size (8.39 ± 3.63 μm) with fructose as carbon source as compared to glucose (2.34 ± 0.44 μm). Fructose syrup was investigated as carbon source under unsterilized conditions and 95.26 ± 1.78% of PHB was produced. Overall, this strain showed the highest PHB contents in halotolerant bacteria.
聚羟基烷酸酯（PHA），如聚（3-羟基丁酸酯）（PHB），已成为石油基塑料的潜在替代品，并且可以通过适当选择已经适应高盐和低温条件的海洋细菌来生产，而无需抗生素处理。因此，本研究旨在筛选和表征从韩国釜山广安里海滩分离出的 13 株PHA 生产微生物菌株。其中，盐单胞菌 YLGW01 用果糖生产出最高量的 PHB（94.6±1.8%（w/w））。有趣的是，与葡萄糖（2.34±0.44μm）相比，盐单胞菌 YLGW01 在用果糖作为碳源时细胞尺寸增加（8.39±3.63μm）。在未灭菌条件下研究了果葡糖浆作为碳源，产生了 95.26±1.78%的 PHB。总的来说，该菌株在耐盐细菌中表现出最高的 PHB 含量。
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