Department of Botany and Microbiology, Faculty of Science, Alexandria University, Egypt.
Marine Biotechnology and Natural Products Extract Laboratory, National Institute of Oceanography and Fisheries, Alexandria, Egypt.
Int J Biol Macromol. 2020 Oct 15;161:1318-1328. doi: 10.1016/j.ijbiomac.2020.07.258. Epub 2020 Aug 2.
Two novel PHA producing bacterial strains were chosen out of 12 strains collected from Mariout salt lakes. Analysis of 16srRNA gene sequence of the two new strains revealed 95.38% and 98.78% similarity to that of Halomonas pacifica and Halomonas salifodiane, respectively. A maximum polymer productivity of 6.9 g/l and 7.1 g/l was recorded by ASL10 and ASL11, respectively. Furthermore, a pH of 7 contributed to the highest polymer production for both strains. Interestingly, both ASL10 and ASL11showed a great ability to tolerate salinity up to 17 g/l NaCL. Moreover, both promising isolates were able to degrade crude oil efficiently by degradation percentages of 69.2% and 67.3% for ASL10 and ASL11, respectively. GCMS, FTIR, NMR, XRD and thermal properties were performed for poly (3 HV-co-3HB) characterization.
从马里奥特盐湖采集的 12 株菌中筛选出两株新型PHA 产生菌。对这两株新菌株的 16srRNA 基因序列分析表明，它们与盐单胞菌（Halomonas pacifica）和盐生盐杆菌（Halomonas salifodiane）的相似度分别为 95.38%和 98.78%。ASL10 和 ASL11 的最大聚合物产率分别为 6.9g/L 和 7.1g/L。此外，pH 值为 7 有利于两种菌株的最高聚合物产量。有趣的是，ASL10 和 ASL11 都表现出很强的耐盐能力，最高耐受盐度可达 17g/L NaCl。此外，这两种有前途的分离物都能够有效地降解原油，ASL10 和 ASL11 的降解率分别为 69.2%和 67.3%。进行了 GCMS、FTIR、NMR、XRD 和热性能分析，以表征聚（3HV-co-3HB）。
