Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju, 61186, Republic of Korea.
Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 61186, Republic of Korea.
Insect Biochem Mol Biol. 2021 Mar;130:103529. doi: 10.1016/j.ibmb.2021.103529. Epub 2021 Jan 21.
Aphids, the major insect pests of agricultural crops, reproduce sexually and asexually depending upon environmental factors such as the photoperiod and temperature. Nuclear receptors, a unique family of ligand-dependent transcription factors, control insect development and growth including morphogenesis, molting, and metamorphosis. However, the structural features and biological functions of the aphid estrogen-related receptor (ERR) are largely unknown. Here, we cloned full-length cDNA encoding the ERR in the green peach aphid, Myzus persicae, (Sulzer) (Hemiptera: Aphididae) (MpERR) and demonstrated that the MpERR modulated glycolytic gene expression and aphid fecundity. The phylogenetic analysis revealed that the MpERR originated in a unique evolutionary lineage distinct from those of hemipteran insects. Moreover, the AF-2 domain of the MpERR conferred nuclear localization and transcriptional activity. The overexpression of the MpERR significantly upregulated the gene expression of rate-limiting enzymes involved in glycolysis such as phosphofructokinase and pyruvate kinase by directly binding to ERR-response elements in their promoters. Moreover, ERR-deficient viviparous female aphids showed decreased glycolytic gene expression and produced fewer offspring. These results suggest that the aphid ERR plays a pivotal role in glycolytic transcriptional control and fecundity.
蚜虫是农业作物的主要害虫，根据光周期和温度等环境因素进行有性和无性繁殖。核受体是一类独特的配体依赖性转录因子家族，控制着昆虫的发育和生长，包括形态发生、蜕皮和变态。然而，蚜虫雌激素相关受体（ERR）的结构特征和生物学功能在很大程度上是未知的。在这里，我们克隆了全长 cDNA 编码绿桃蚜，桃蚜，（苏尔泽）（半翅目：蚜科）（MpERR），并证明 MpERR 调节糖酵解基因的表达和蚜虫的繁殖力。系统发育分析表明，MpERR 起源于一个独特的进化谱系，与半翅目昆虫的进化谱系不同。此外，MpERR 的 AF-2 结构域赋予核定位和转录活性。MpERR 的过表达通过直接结合到它们启动子中的 ERR 反应元件，显著地上调了参与糖酵解的限速酶如磷酸果糖激酶和丙酮酸激酶的基因表达。此外，ERR 缺陷的胎生雌性蚜虫表现出糖酵解基因表达降低，并产生较少的后代。这些结果表明，蚜虫 ERR 在糖酵解转录控制和繁殖力中起着关键作用。
