Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
Department of Microbiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
J Virol Methods. 2021 Apr;290:114073. doi: 10.1016/j.jviromet.2021.114073. Epub 2021 Jan 21.
The human T-lymphotropic virus type 1 (HTLV-1) can cause ATL or TSP. This study evaluates the prevalence of HTLV-1 infection in blood donors in Golestan province. The study was conducted among 4226 blood donors and ELISA test was performed for the initial HTLV-1 screening. Reactive samples were confirmed by Western blot and Electrochemiluminescence tests. Then recalling donors with reactive results was done and genomic DNA from the new sample was extracted and tested using the Nested PCR method and phylogenetic analysis was performed. At first, 8 samples were reactive with ELISA test and 4 samples were confirmed with western blot, Electrochemiluminescence and Nested PCR tests.The sequences of isolates was related to the HTLV-1 virus and subtype a (cosmopolitan) subgroup A.The prevalence of HTLV-1 virus in Golestan province was about 0.09 %.The genotype of virus isolates had a common ancestor with isolates of the Khorasan region.
人类 T 淋巴细胞白血病病毒 1 型（HTLV-1）可引起 ATL 或 TSP。本研究评估了戈勒斯坦省献血者中 HTLV-1 感染的流行情况。该研究在 4226 名献血者中进行，采用 ELISA 试验进行 HTLV-1 初步筛查。对反应性样本采用 Western blot 和电化学发光试验进行确认。然后召回有反应性结果的献血者，并从新样本中提取基因组 DNA，采用巢式 PCR 方法进行检测，并进行系统发育分析。首先，8 份样本的 ELISA 检测呈阳性，4 份样本的 Western blot、电化学发光和巢式 PCR 检测呈阳性。分离株的序列与 HTLV-1 病毒和亚型 a（世界性）A 亚群相关。戈勒斯坦省 HTLV-1 病毒的流行率约为 0.09%。病毒分离株的基因型与霍拉桑地区的分离株有共同的祖先。
