Azarpazhooh Mahmoud Reza, Hasanpour Kazem, Ghanbari Mohsen, Rezaee S A Rahim, Mashkani Baratali, Hedayati-Moghaddam Mohammad Reza, Valizadeh Narges, Farid Hosseini Reza, Foroghipoor Mohsen, Soltanifar Azadeh, Sahebari Maryam, Azadmanesh Keyhan, Hassanshahi Gholahossein, Rafatpanah Houshang
Department of Neurology, Mashhad University of Medical Sciences, Iran.
AIDS Res Hum Retroviruses. 2012 Sep;28(9):1095-1101. doi: 10.1089/AID.2011.0248. Epub 2012 Mar 19.
Human T-lymphotropic virus type 1 (HTLV-I) is an important global health problem in the world mainly in the endemic areas of HTLV-I infection. It was previously reported that Mashhad, in northeastern Iran, is a new endemic region of HTLV-I. The aim of this study was to examine the prevalence and phylogenetic analysis of HTLV-I in Sabzevar, located in the southeast of Mashhad. In this cross-sectional study 1445 individuals were selected by multistage cluster sampling. Serum samples were screened for anti-HTLV-I antibody using enzyme-linked immunosorbent assay (ELISA); all of the ELISA-positive samples were confirmed by polymerase chain reaction (PCR). Long terminal repeat (LTR) sequencing was carried out to determine the type of HTLV-I in Sabzevar. In the primary screening by ELISA, 26/1445 (1.8%) of those sampled were reactive for HTLV-I antibody. Twenty-four out of 26 samples were confirmed HTLV-I infection by PCR (24/1445). The overall prevalence of HTLV-I infection in Sabzevar is 1.66%. The prevalence of the virus infection in men and women was 2.42% (11/455) and 1.31% (13/989), respectively. Seroprevalence was associated with age, increasing significantly among those older than 30 years (p=0.015), and a history of surgery (p=0.002), imprisonment (p=0.018), and hospitalization (p=0.005). Three out of 24 positive HTLV-I samples were selected for sequencing and phylogenetic analysis of LTR. The results showed that HTLV-I in Sabzevar belonged to the cosmopolitan subtype. The present study showed Sabzevar is a new endemic area for HTLV-I infection. Our study emphasizes that systemic HTLV-I screening of blood donors in Sabzevar and other cities in Khorasan province is important and should be taken into account.
人类嗜T淋巴细胞病毒1型（HTLV-I）是一个全球性的重要健康问题，主要存在于HTLV-I感染的流行地区。此前有报道称，伊朗东北部的马什哈德是HTLV-I的一个新流行地区。本研究的目的是调查位于马什哈德东南部的萨卜泽瓦尔市HTLV-I的流行情况并进行系统发育分析。在这项横断面研究中，通过多阶段整群抽样选取了1445名个体。使用酶联免疫吸附测定（ELISA）对血清样本进行抗HTLV-I抗体筛查；所有ELISA阳性样本均通过聚合酶链反应（PCR）进行确认。进行长末端重复序列（LTR）测序以确定萨卜泽瓦尔市HTLV-I的类型。在ELISA的初步筛查中，1445名抽样者中有26人（1.8%）的HTLV-I抗体呈阳性反应。26份样本中有24份通过PCR确认感染了HTLV-I（24/1445）。萨卜泽瓦尔市HTLV-I感染的总体患病率为1.66%。该病毒在男性和女性中的感染率分别为2.42%（11/455）和1.31%（13/989）。血清阳性率与年龄相关，在30岁以上人群中显著增加（p=0.015），并且与手术史（p=0.002）、监禁史（p=0.018）和住院史（p=0.005）有关。从24份HTLV-I阳性样本中选取3份进行LTR测序和系统发育分析。结果表明，萨卜泽瓦尔市的HTLV-I属于世界流行亚型。本研究表明，萨卜泽瓦尔市是HTLV-I感染的一个新流行地区。我们的研究强调，对萨卜泽瓦尔市和霍拉桑省其他城市的献血者进行系统性HTLV-I筛查很重要，应予以考虑。
