Bordeaux INP, CBMN, UMR 5248, F-33600, Pessac, France.
CNRS, CBMN, UMR 5248, F-33600, Pessac, France.
Protein Expr Purif. 2021 May;181:105830. doi: 10.1016/j.pep.2021.105830. Epub 2021 Jan 22.
Hsp12 is a small heat shock protein of Saccharomyces cerevisiae upregulated in response to various stresses. Non recombinant Hsp12 has been purified and characterized. Using circular dichroism (CD), Isothermal Titration Calorimetry (ITC) and Differential Scanning Calorimetry (DSC), it has been demonstrated that the native Hsp12 is monomeric and intrinsically disordered (IDP). Hsp12 gains in structure in the presence of specific lipids (PiP). The helical form binds to liposomes models membrane with high affinity, leading to their rigidification. These results suggest that hydrophobic and ionic interactions are involved. Hsp12 is most likely a membrane chaperone expressed during stresses in Saccharomyces cerevisiae.
Hsp12 是酿酒酵母中的一种小热休克蛋白，可响应各种应激而上调。已对非重组 Hsp12 进行了纯化和表征。通过圆二色性（CD）、等温热滴定（ITC）和差示扫描量热法（DSC），证明天然 Hsp12 是单体且无规卷曲（IDP）。在特定脂质（PiP）存在下，Hsp12 获得结构。螺旋形式与脂质体模型膜具有高亲和力结合，导致其刚性增加。这些结果表明涉及疏水和离子相互作用。Hsp12 很可能是酿酒酵母应激时表达的一种膜伴侣。
