Department of Advanced Science and Technology, Japan Advanced Institute of Science and Technology, Asahidai 1-1, Nomi, Ishikawa 923-1292, Japan.
Department of Advanced Science and Technology, Japan Advanced Institute of Science and Technology, Asahidai 1-1, Nomi, Ishikawa 923-1292, Japan.
Bioorg Med Chem Lett. 2021 Mar 1;35:127812. doi: 10.1016/j.bmcl.2021.127812. Epub 2021 Jan 22.
Nucleobase editing is a powerful tool in genetic disease therapy. We have reported the photochemical transition of cytosine to uracil using an ultrafast DNA photo-cross-linking. In this study, we used cytosine derivatives such as methylcytosine, hydroxymethylcytosine, and trifluoromethylcytosine to evaluate the effect of 5-position substitution of cytosine on deamination. The conversion of cytosine to uracil was the fastest, and the conversion of trifluoromethylcytosine to trifluoromethyluracil was the slowest. The order was correlated with the hydrophilicity of the double strand containing these cytosine derivatives.
碱基编辑是一种用于治疗遗传疾病的强大工具。我们曾报道过使用超快 DNA 光交联技术实现胞嘧啶向尿嘧啶的光化学转变。在这项研究中，我们使用胞嘧啶衍生物，如甲基胞嘧啶、羟甲基胞嘧啶和三氟甲基胞嘧啶，来评估胞嘧啶 5 位取代对脱氨的影响。结果表明，胞嘧啶向尿嘧啶的转化最快，而三氟甲基胞嘧啶向三氟甲基尿嘧啶的转化最慢。这种顺序与含有这些胞嘧啶衍生物的双链的亲水性相关。
