Takahashi H, Sasaki F, Hata Y, Une Y, Inoue K, Uchino J
1st Dept. of Surgery, School of Medicine, Hokkaido University.
Gan To Kagaku Ryoho. 1988 Mar;15(3):437-42.
Chemosensitivity of liver cell carcinoma was studied by subrenal capsule assay. The method of assay was based on Bogden's one, but the antitumor activity was evaluated by tumor growth inhibition rate (TG-IR). The anticancer agent with more than 50% TG-IR was judged as positive in the chemosensitivity test. Of 3 human hepatoma cell lines transplanted in the subcutaneous space of nude mice, all of 3 were evaluable. The positive rates of ADR, MMC, CDDP, 5-FU and CPA were 66.7%, 100%, 66.7%, 100% and 0%, respectively. Of 24 patients who provided fresh tumor specimens for the assay, 12 (50%) were evaluable. The positive rates of ADR, MMC, CDDP, 5-FU and CPA were 25%, 16.7%, 16.7%, 33.3% and 8.3%, respectively. Our study suggested that 5-FU, MMC and ADR were comparatively active against the hepatoma cell, CDDP was less active than these 3 agents, CPA was inactive. These results seem to justify the use of current anticancer agents against hepatic cell carcinoma and indicate the usefulness of SRC assay for selecting chemotherapeutic agents against liver cell carcinoma.
采用肾包膜下接种法研究肝癌细胞的化学敏感性。该检测方法基于博登的方法，但通过肿瘤生长抑制率（TG-IR）评估抗肿瘤活性。在化学敏感性试验中，TG-IR超过50%的抗癌药物被判定为阳性。在接种于裸鼠皮下的3种人肝癌细胞系中，所有3种均可供评估。阿霉素（ADR）、丝裂霉素（MMC）、顺铂（CDDP）、5-氟尿嘧啶（5-FU）和环磷酰胺（CPA）的阳性率分别为66.7%、100%、66.7%、100%和0%。在为该检测提供新鲜肿瘤标本的24例患者中，12例（50%）可供评估。ADR、MMC、CDDP、5-FU和CPA的阳性率分别为25%、16.7%、16.7%、33.3%和8.3%。我们的研究表明，5-FU、MMC和ADR对肝癌细胞的活性相对较高，CDDP的活性低于这3种药物，CPA无活性。这些结果似乎证明了目前使用的抗癌药物对肝癌的有效性，并表明肾包膜下接种法在选择抗肝癌化疗药物方面的有用性。
