Kikuchi H, Asamura M, Gamoh M, Matsushita T, Chen D Y, Wakui A, Takahashi T
Dept. of Clin. Cancer Chemotherapy, Tohoku University.
Gan To Kagaku Ryoho. 1988 Mar;15(3):499-504.
Six-day SRCA using normal mice developed by Bogden et al. is a promising in vivo chemosensitivity test. However, this method has a problem on the influence of the host reaction. We compared the tumor growth kinetics and host reaction between normal and nude mice. The tumor diameter increased until day 6 in normal and day 16 in nude mice, but the histological finding revealed many host reactive cells and few viable tumour cells on day 6 in normal mice, and well preserved tumour cells on day 16 in nude mice. These results were supported by flow cytometrical analysis. Autoradiogram using 3H-TdR showed a recovery of labeling index to the steady label by day 1. This index was similar between normal and nude mice. When antitumor activity of adriamycin, cisplatin or mitomycin C was compared with nude mice system, the order of effectiveness was the same as the system using nude mice implanted tumor cells subcutaneously and given the drugs intraperitoneally, but different in normal mice.
Bogden等人研发的使用正常小鼠的六日短期抗癌药敏试验（SRCA）是一种很有前景的体内化疗敏感性测试方法。然而，该方法存在宿主反应影响方面的问题。我们比较了正常小鼠和裸鼠之间的肿瘤生长动力学及宿主反应。肿瘤直径在正常小鼠中到第6天增大，在裸鼠中到第16天增大，但组织学检查发现，正常小鼠在第6天时存在许多宿主反应性细胞且存活肿瘤细胞很少，而裸鼠在第16天时肿瘤细胞保存良好。这些结果得到了流式细胞仪分析的支持。使用³H-胸腺嘧啶核苷（³H-TdR）的放射自显影片显示，标记指数到第1天时恢复到稳定标记水平。该指数在正常小鼠和裸鼠之间相似。当将阿霉素、顺铂或丝裂霉素C与裸鼠系统的抗肿瘤活性进行比较时，有效性顺序与皮下植入肿瘤细胞并腹腔注射药物的裸鼠系统相同，但在正常小鼠中不同。
