Key Laboratory of Environmental Medicine Engineering of Ministry of Education, Medical School, Southeast University, Nanjing, 210009, China.
Key Laboratory of Environmental Medicine Engineering of Ministry of Education, Medical School, Southeast University, Nanjing, 210009, China; Shenzhen Ruipuxun Academy for Stem Cell & Regenerative Medicine, Shenzhen, 518122, China; College of Biology and Food Engineering, Chongqing Three Gorges University, Wanzhou, 404100, China.
Chemosphere. 2021 Jun;273:129686. doi: 10.1016/j.chemosphere.2021.129686. Epub 2021 Jan 18.
Ion channels on cytoplasmic membrane function to sense various environmental stimuli. We here determined the changes of genes encoding ion channels in Caenorhabditis elegans after exposure to polystyrene nanoparticles (PS-NPs). Exposure to 1-1000 μg/L PS-NPs could increase expressions of egl-19, mec-10, trp-4, trp-2, tax-4, cca-1, unc-2, and unc-93, and decrease the expressions of cng-3, mec-6, ocr-2, deg-1, exc-4, kvs-1, and eat-2. Among these 15 ion channel genes, RNAi knockdown of cng-3 or eat-2 caused resistance to PS-NPs toxicity and RNAi knockdown of egl-19, cca-1, tax-4, or unc-93 induced susceptibility to PS-NPs toxicity, suggesting that cng-3, eat-2, egl-19, cca-1, tax-4, and unc-93 were involved in the control of PS-NPs toxicity. EGL-19 and CCA-1 functioned in intestinal cells to control PS-NPs toxicity, and CNG-3, EAT-2, EGL-19, TAX-4, and UNC-93 functioned in neuronal cells to control PS-NPs. Moreover, in intestinal cells of PS-NPs exposed worms, cca-1 RNAi knockdown decreased elt-2 expression, and egl-19 RNAi knockdown decreased daf-16 and elt-2 expressions. In neuronal cells of PS-NPs exposed worms, eat-2 RNAi knockdown increased jnk-1, mpk-1, and dbl-1 expressions, unc-93 RNAi knockdown decreased mpk-1 and daf-7 expressions, and tax-4 RNAi knockdown decreased jnk-1 and daf-7 expressions. Therefore, two molecular networks mediated by ion channels in intestinal cells and neuronal cells were dysregulated by PS-NPs exposure in C. elegans. Our data suggested that the dysregulation in expressions of these ion channels mediated a protective response to PS-NPs in the range of μg/L in worms.
细胞质膜上的离子通道可感知各种环境刺激。我们在此确定了暴露于聚苯乙烯纳米颗粒（PS-NPs）后秀丽隐杆线虫编码离子通道的基因变化。暴露于 1-1000μg/L PS-NPs 可增加 egl-19、mec-10、trp-4、trp-2、tax-4、cca-1、unc-2 和 unc-93 的表达，并降低 cng-3、mec-6、ocr-2、deg-1、exc-4、kvs-1 和 eat-2 的表达。在这 15 个离子通道基因中，cng-3 或 eat-2 的 RNAi 敲低导致对 PS-NPs 毒性的抗性，而 egl-19、cca-1、tax-4 或 unc-93 的 RNAi 敲低则诱导对 PS-NPs 毒性的敏感性，表明 cng-3、eat-2、egl-19、cca-1、tax-4 和 unc-93 参与了 PS-NPs 毒性的控制。EGL-19 和 CCA-1 在肠细胞中发挥作用以控制 PS-NPs 毒性，而 CNG-3、EAT-2、EGL-19、TAX-4 和 UNC-93 在神经元细胞中发挥作用以控制 PS-NPs。此外，在暴露于 PS-NPs 的线虫的肠细胞中，cca-1 RNAi 敲低降低了 elt-2 的表达，而 egl-19 RNAi 敲低降低了 daf-16 和 elt-2 的表达。在暴露于 PS-NPs 的线虫的神经元细胞中，eat-2 RNAi 敲低增加了 jnk-1、mpk-1 和 dbl-1 的表达，unc-93 RNAi 敲低降低了 mpk-1 和 daf-7 的表达，而 tax-4 RNAi 敲低降低了 jnk-1 和 daf-7 的表达。因此，两个由离子通道介导的分子网络在 PS-NPs 暴露后在秀丽隐杆线虫中被扰乱。我们的数据表明，在μg/L 范围内，这些离子通道表达的失调介导了线虫对 PS-NPs 的保护反应。
