College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Ecotoxicol Environ Saf. 2021 Apr 1;212:111976. doi: 10.1016/j.ecoenv.2021.111976. Epub 2021 Jan 28.
Intestinal barrier plays a crucial function during the response to polystyrene nanoparticles (PS-NPs) in nematode Caenorhabditis elegans. Long non-coding RNAs (lncRNAs) are involved in the control of various biological processes, including stress response. We here used C. elegans to determine intestinal lncRNAs dysregulated by PS-NPs (1-100 μg/L). In intestine of PS-NPs exposed worms, we found four lncRNAs (linc-61, linc-50, linc-9, and linc-2) in response to PS-NPs and with the function in controlling PS-NPs toxicity. The alteration in expressions of these four intestinal lncRNAs reflected a protective response to PS-NPs exposure. During the response to PS-NPs, limited number of transcriptional factors functioned as the downstream targets of these four lncRNAs. linc-2 acted upstream of DAF-16, linc-9 acted upstream of NHR-77, linc-50 functioned upstream of DAF-16, and linc-61 regulated the functions of DAF-16, DVE-1, and FKH-2 to control PS-NPs toxicity. The obtained data demonstrated the important role of lncRNAs in intestinal barrier to mediate a protective response to PS-NPs exposure at low concentrations.
肠道屏障在秀丽隐杆线虫对聚苯乙烯纳米颗粒（PS-NPs）的反应中起着至关重要的作用。长非编码 RNA（lncRNA）参与控制各种生物过程，包括应激反应。我们在这里使用秀丽隐杆线虫来确定受 PS-NPs（1-100μg/L）调控的肠道 lncRNA。在暴露于 PS-NPs 的线虫的肠道中，我们发现了四个响应 PS-NPs 并具有控制 PS-NPs 毒性功能的 lncRNA（linc-61、linc-50、linc-9 和 linc-2）。这四个肠道 lncRNA 的表达变化反映了对 PS-NPs 暴露的保护反应。在对 PS-NPs 的反应中，少数转录因子作为这四个 lncRNA 的下游靶标发挥作用。linc-2 作用于 DAF-16 的上游，linc-9 作用于 NHR-77 的上游，linc-50 作用于 DAF-16 的上游，而 linc-61 调节 DAF-16、DVE-1 和 FKH-2 的功能以控制 PS-NPs 的毒性。这些数据表明，lncRNA 在肠道屏障中起着重要作用，可在低浓度下介导对 PS-NPs 暴露的保护反应。
