Medical School, Southeast University, Nanjing 210009, China.
Medical School, Southeast University, Nanjing 210009, China.
J Hazard Mater. 2021 Jun 5;411:125035. doi: 10.1016/j.jhazmat.2020.125035. Epub 2021 Jan 6.
Caenorhabditis elegans is a useful animal model to assess nanoplastic toxicity. Using polystyrene nanoparticles (PS-NPs) as the example of nanoplastics, we found that exposure to PS-NPs (1-100 μg/L) from L1-larvae for 6.5 days increased expression of cbp-1 encoding an acetyltransferase. The susceptibility to PS-NPs toxicity was observed in cbp-1(RNAi) worms, suggesting that CBP-1-mediated histone acetylation regulation reflects a protective response to PS-NPs. The functions of CBP-1 in intestine, neurons, and germline were required for formation of this protective response. In intestinal cells, CBP-1 controlled PS-NPs toxicity by modulating functions of insulin and p38 MAPK signaling pathways. In neuronal cells, CBP-1 controlled PS-NPs toxicity by affecting functions of DAF-7/TGF-β and JNK MAPK signaling pathways. In germline cells, CBP-1 controlled PS-NPs toxicity by suppressing NHL-2 activity, and NHL-2 further regulated PS-NPs toxicity by modulating insulin communication between germline and intestine. Therefore, our data suggested that the CBP-1-mediated histone acetylation regulation in certain tissues is associated with the induction of protective response to PS-NPs in C. elegans.
秀丽隐杆线虫是评估纳米塑料毒性的有用动物模型。我们以聚苯乙烯纳米颗粒 (PS-NPs) 为例，发现从 L1 幼虫期开始暴露于 PS-NPs（1-100μg/L）6.5 天会增加编码乙酰转移酶的 cbp-1 的表达。在 cbp-1（RNAi）蠕虫中观察到对 PS-NPs 毒性的易感性，表明 CBP-1 介导的组蛋白乙酰化调节反映了对 PS-NPs 的保护反应。CBP-1 在肠道、神经元和生殖系中的功能对于形成这种保护反应是必需的。在肠细胞中，CBP-1 通过调节胰岛素和 p38 MAPK 信号通路的功能来控制 PS-NPs 毒性。在神经元细胞中，CBP-1 通过影响 DAF-7/TGF-β 和 JNK MAPK 信号通路的功能来控制 PS-NPs 毒性。在生殖细胞中，CBP-1 通过抑制 NHL-2 活性来控制 PS-NPs 毒性，而 NHL-2 通过调节生殖细胞和肠道之间的胰岛素通讯进一步调节 PS-NPs 毒性。因此，我们的数据表明，CBP-1 介导的组蛋白乙酰化调节与秀丽隐杆线虫中对 PS-NPs 的诱导保护反应有关。
