Human Eosinophil Section, Laboratory of Parasitic Diseases, National Institute of Allergy, Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2021;2241:27-35. doi: 10.1007/978-1-0716-1095-4_3.
Eosinophils are granulocytes involved mainly in allergic inflammation and parasitic responses and constitute 1-5% of the circulating leukocytes in human healthy subjects. New immunotherapies targeting eosinophils have been developed and evaluated recently, and the availability of animal models that could mimic human eosinophil responses is important to consider. Differences in eosinophil biology and pathogenesis between humans and murine models have limited their utility in some settings. Isolation of viable eosinophils from rhesus macaque blood suitable for ex vivo and in vitro experimentation could provide a valuable tool for the study of eosinophil-targeted therapies and for the exploration of eosinophilic associated responses. Here, a new technique for the isolation of human eosinophils from rhesus macaque blood by negative selection from whole blood is described.
嗜酸性粒细胞是主要参与过敏炎症和寄生虫反应的粒细胞，占健康人类循环白细胞的 1-5%。最近已经开发和评估了针对嗜酸性粒细胞的新免疫疗法，考虑到能够模拟人类嗜酸性粒细胞反应的动物模型的可用性非常重要。人类和鼠模型之间嗜酸性粒细胞生物学和发病机制的差异限制了它们在某些情况下的应用。从恒河猴血液中分离出适合离体和体外实验的活嗜酸性粒细胞，可以为研究针对嗜酸性粒细胞的治疗方法和探索与嗜酸性粒细胞相关的反应提供有价值的工具。在这里，描述了一种从恒河猴血液中通过阴性选择从全血中分离人嗜酸性粒细胞的新技术。
