Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2021;2241:49-58. doi: 10.1007/978-1-0716-1095-4_5.
Flow cytometry is a critical tool that can be employed to detect unique cells and to isolate cells from tissues based on their antigen profiles. While mouse eosinophils can be readily detected by one or more distinct antigen profiles, many of these strategies do not result in accurate eosinophil counts. We present here our basic protocol, which permits quantitative detection of eosinophils and isolation of eosinophils from bone marrow, spleen, and lung tissue of allergen-challenged wild-type and unchallenged IL5 transgenic mice. With small protocol variations, eosinophils can be isolated from small intestines and muscle tissue, the latter from infiltrates characteristic of muscular dystrophy (mdx) mice.
流式细胞术是一种重要的工具，可以用于检测独特的细胞，并根据其抗原特征从组织中分离细胞。虽然小鼠嗜酸性粒细胞可以通过一种或多种独特的抗原特征来轻易检测到，但许多这些策略并不能得到准确的嗜酸性粒细胞计数。我们在这里介绍了我们的基本方案，该方案允许定量检测和从过敏原刺激的野生型和未刺激的 IL5 转基因小鼠的骨髓、脾脏和肺部组织中分离嗜酸性粒细胞。通过微小的方案变化，可以从小肠和肌肉组织中分离嗜酸性粒细胞，后者来自肌肉营养不良症（mdx）小鼠的浸润特征。
