Centre de Recherche, Institut Universitaire de Cardiologie et de Pneumologie de Québec, Quebec City, QC, Canada.
Methods Mol Biol. 2021;2241:59-74. doi: 10.1007/978-1-0716-1095-4_6.
Eosinophilia is a hallmark of allergic airway inflammation, and eosinophils represent an integral effector leukocyte through their release of various granule-stored cytokines and proteins. Numerous mouse models have been developed to mimic clinical disease and they have been instrumental in furthering our understanding of the role of eosinophils in disease. Most of these models consist of intranasal (i.n.) administration of antigenic proteases including papain and house dust mite (HDM) or the neo-antigen ovalbumin, with a resulting Th2-biased immune response and airway eosinophilia. These models have been particularly informative when combined with the numerous transgenic mice available that modulate eosinophil frequency or the mechanisms involved in their migration. Here, we describe the current models of allergic airway inflammation and outline some of the transgenic mice available to study eosinophil disease.
嗜酸性粒细胞增多症是过敏气道炎症的标志，嗜酸性粒细胞通过释放各种颗粒储存的细胞因子和蛋白质，代表了一种不可或缺的效应白细胞。已经开发了许多小鼠模型来模拟临床疾病，它们在进一步了解嗜酸性粒细胞在疾病中的作用方面发挥了重要作用。这些模型大多数包括鼻内（i.n.）给予抗原蛋白酶，如木瓜蛋白酶和屋尘螨（HDM）或新抗原卵清蛋白，导致 Th2 偏向的免疫反应和气道嗜酸性粒细胞增多症。当与可调节嗜酸性粒细胞频率或其迁移所涉及的机制的众多转基因小鼠结合使用时，这些模型特别有启发性。在这里，我们描述了过敏气道炎症的当前模型，并概述了一些可用于研究嗜酸性粒细胞疾病的转基因小鼠。
