Department of Clinical Microbiology and Immunology, The Sackler School of Medicine, Tel-Aviv University, Ramat Aviv, Israel.
Methods Mol Biol. 2021;2241:89-97. doi: 10.1007/978-1-0716-1095-4_8.
Eosinophils are bone marrow-derived cells that differentiate in the bone marrow and migrate into the peripheral blood primarily under the regulation of interleukin (IL)-5. Despite the fact that eosinophils have been mostly studied in the context of allergic inflammatory diseases, eosinophils accumulate in multiple tumors. In fact, recent data highlight key anti-tumorigenic activities for eosinophils. Thus, developing simple assays that will dissect the interactions between eosinophils and tumor cells is important since these assays will provide tools to study eosinophils in the tumor microenvironment. In this chapter, we provide detailed methods for isolating eosinophils from Il5 transgenic mice. Furthermore, we provide methodology to assess eosinophil chemotaxis in response to tumor-secreted factors. Finally, we describe a co-culture system of eosinophils and tumor cells aimed to determine the cytotoxic capabilities of eosinophils.
嗜酸性粒细胞是骨髓来源的细胞，在骨髓中分化，并主要在白细胞介素（IL）-5 的调节下迁移到外周血中。尽管嗜酸性粒细胞在过敏性炎症性疾病的背景下已被广泛研究，但它们在多种肿瘤中积累。事实上，最近的数据强调了嗜酸性粒细胞的关键抗肿瘤活性。因此，开发能够剖析嗜酸性粒细胞与肿瘤细胞之间相互作用的简单检测方法非常重要，因为这些检测方法将为研究肿瘤微环境中的嗜酸性粒细胞提供工具。在本章中，我们提供了从 Il5 转基因小鼠中分离嗜酸性粒细胞的详细方法。此外，我们还提供了评估嗜酸性粒细胞对肿瘤分泌因子趋化性的方法。最后，我们描述了一种嗜酸性粒细胞和肿瘤细胞的共培养系统，旨在确定嗜酸性粒细胞的细胞毒性能力。
