Grevers G, Heinzmann U
Department of Otorhinolaryngology, University of Munich, Klinikum Grosshadern, Federal Republic of Germany.
Arch Otorhinolaryngol. 1988;244(6):363-6. doi: 10.1007/BF00497466.
Current histological and transmission electron microscopic techniques are insufficient for studying all different aspects of vascular morphology. As a result, we have used scanning electron microscopy (SEM) to examine nasal blood vessels. In our present studies the nasal septa of 15 rabbits were fixed by vascular perfusion, dried by the critical point method and coated with gold for SEM studies. Lower magnifications of specimens using this method show the tissue arrangement in the nasal septum. In higher magnifications the different vessels can be distinguished by their morphological features. The course of the vascular structures can be observed as well as the junctions between the different vessels. Our results indicate that different morphological techniques must be combined to understand the endonasal vasculature.
当前的组织学和透射电子显微镜技术不足以研究血管形态的所有不同方面。因此,我们使用扫描电子显微镜(SEM)来检查鼻血管。在我们目前的研究中,对15只兔子的鼻中隔进行血管灌注固定,通过临界点干燥法干燥,然后进行镀金处理以用于扫描电子显微镜研究。使用这种方法对标本进行低倍放大可显示鼻中隔中的组织排列。在高倍放大下,可以通过不同血管的形态特征将它们区分开来。可以观察到血管结构的走向以及不同血管之间的连接。我们的结果表明,必须结合不同的形态学技术来了解鼻内血管系统。