Grevers G, Heinzmann U
HNO-Klinik und Poliklinik, Universität München.
Laryngol Rhinol Otol (Stuttg). 1988 Oct;67(10):526-31.
Histological and transmission-electron microscopical studies alone cannot answer all questions regarding the microcirculation of the nasal mucosa. In the present study additional morphological techniques were used to demonstrate the course of the different parts of this peculiar vascular network. Using the "injection technique", the course of every single vessel of the nasal septum could be shown, but the character or size of these vascular structures was not easy to identify. Scanning electron microscopic investigations were able to verify vascular junctions of all sizes as well as intraluminal aspects of different vessels and the relations between all parts of this endonasal circulatory system. Lower magnifications also showed the tissue arrangement in the nasal septum. In higher magnifications vessels of arterial or venous character could be identified by distinct morphological features. Additional studies were carried out using vascular corrosion casts. On studying these structures, the entire course of the different parts of the endonasal vascular system could be observed. In addition, the arrangement of the different capillary networks of the nasal system, mentioned by several earlier investigators, could be demonstrated without doubt. This technique also allowed to distinguish between arteries and veins by the appearance of their endothelial cell nuclei. Summing up our findings it can be pointed out that all techniques used in this study are useful to understand the complicated angioarchitecture of the nasal mucosa. This evaluation may also be regarded as an extension of our earlier histological and ultrastructural investigations in this field.
仅靠组织学和透射电子显微镜研究无法回答所有关于鼻黏膜微循环的问题。在本研究中,采用了额外的形态学技术来展示这个特殊血管网络不同部分的走向。使用“注射技术”,可以显示鼻中隔每一根血管的走向,但这些血管结构的特征或大小并不容易识别。扫描电子显微镜研究能够证实各种大小的血管连接以及不同血管的管腔内情况,还有这个鼻内循环系统各部分之间的关系。低倍放大还显示了鼻中隔的组织排列。在高倍放大下,可以通过明显的形态特征识别出动脉或静脉性质的血管。还使用血管铸型进行了额外的研究。在研究这些结构时,可以观察到鼻内血管系统不同部分的整个走向。此外,几位早期研究者提到的鼻系统不同毛细血管网的排列也能够得到明确证实。这项技术还能通过内皮细胞核的外观区分动脉和静脉。总结我们的研究结果可以指出,本研究中使用的所有技术都有助于理解鼻黏膜复杂的血管结构。这种评估也可被视为我们此前在该领域进行的组织学和超微结构研究的延伸。