Diatom Research Laboratory, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh 201313, India.
Diatom Research Laboratory, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh 201313, India.
Bioresour Technol. 2021 Apr;325:124708. doi: 10.1016/j.biortech.2021.124708. Epub 2021 Jan 14.
Diatoms are one of the most heterogeneous eukaryotic plankton known for regulating earth's biogeochemical cycles and maintaining the marine ecosystems ever since the late Eocene epoch. The advent of multidisciplinary omics approach has both epitomized and revolutionized the nature of their chimeric genetic toolkit, ecophysiology, and metabolic adaptability as well as their interaction with other communities. In addition, advanced functional annotation of transcriptomic and proteomic data using cutting edge bioinformatics tools together with high-resolution genome-scale mathematical modeling has effectively proven as the catapult in solving genetic bottlenecks in microbial as well as diatom exploration. In this review, a corroborative summation of the robust work done in manipulating, engineering, and sequencing of the diatom genomes besides underpinning the holistic application of omics in transcription and translation has been discussed in order to shrewd their multifarious novel potential in the field of biotechnology and provide an insight into their dynamic evolutionary relevance.
硅藻是最具异质性的真核浮游生物之一，自晚始新世以来，它们通过调节地球的生物地球化学循环和维持海洋生态系统而闻名。多学科组学方法的出现不仅概括了它们嵌合遗传工具包、生理生态和代谢适应性的本质，而且还概括了它们与其他群落的相互作用。此外，使用最先进的生物信息学工具对转录组和蛋白质组数据进行高级功能注释，以及高分辨率的基于基因组的数学建模，已被证明是解决微生物和硅藻探索中遗传瓶颈的有效方法。在这篇综述中，我们讨论了在硅藻基因组的操纵、工程和测序方面所做的扎实工作的综合总结，同时还支持了组学在转录和翻译中的整体应用，以挖掘它们在生物技术领域的多种新潜力，并深入了解它们的动态进化相关性。
