Research Center for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing, 100083, PR China.
Research Center for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing, 100083, PR China.
Biosens Bioelectron. 2020 Jun 15;158:112185. doi: 10.1016/j.bios.2020.112185. Epub 2020 Apr 2.
Ultrasound as a biocompatible and powerful approach has been advanced in biotechnology. Here we present an acoustic microchip integrating modification and detection for in-situ analysis. Such microchip employs two pairs of piezoelectric transducers (PZTs) for acoustic field generation and a polydimethylsiloxane (PDMS) microcavity on a polyethylene terephthalate (PET) substrate for producing microparticle array. The applying of acoustic field results in rapidly forming microparticle array by adjusting the inputting frequency and voltage. In-situ modification and detection are accelerated due to the dynamic ultrasonic streaming around the ultrasound induced microparticle array. Such array also benefits from reducing the detection errors by coupling of multiple points. With this strategy, biomarkers (e.g. miRNA) can be enriched, and achieve in-situ modification and detection via simple two steps with excellent specificity. After the detection, samples are regained from the output channel by releasing the acoustic field, which is benefit for further analysis. Such integrated modification and detection acoustic microchip shows great potential in visual in-situ analysis and enriching ultratrace biomarkers for clinical diagnosis.
超声作为一种生物相容性强且功能强大的方法，已在生物技术中得到了广泛应用。在这里，我们提出了一种集成修饰和检测功能的声学微芯片，用于原位分析。该微芯片采用两对压电换能器（PZT）用于声场产生，以及聚二甲基硅氧烷（PDMS）微腔用于在聚对苯二甲酸乙二醇酯（PET）基底上产生微颗粒阵列。通过调整输入频率和电压，可以应用声场快速形成微颗粒阵列。由于超声诱导的微颗粒阵列周围的动态超声流，原位修饰和检测得以加速。这种微颗粒阵列还通过多点耦合获益于减少检测误差。通过这种策略，可以富集生物标志物（例如 miRNA），并通过简单的两步原位修饰和检测实现，具有出色的特异性。检测后，通过释放声场从输出通道中回收样品，这有利于进一步分析。这种集成修饰和检测声学微芯片在可视化原位分析和富集痕量生物标志物用于临床诊断方面显示出巨大的潜力。
