Yukawa Hiroshi, Baba Yoshinobu
Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University.
ImPACT Research Center for Advanced Nanobiodevices, Nagoya University.
Anal Sci. 2018;34(5):525-532. doi: 10.2116/analsci.17R005.
Quantum dots (QDs) have excellent fluorescence properties in comparison to traditional fluorescence probes. Thus, the optical application of QDs is rapidly expanding to each field of analytical chemistry. In this review paper, we reviewed the application of QDs to regenerative medicine, especially stem cell transplantation therapy. The labeling of stem cells using QDs composed of semiconductor materials in combination with a chemical substance, poly-cationic liposome and cell penetrating peptide is reported. In addition, the influence of QD labeling on the pluripotency of stem cells is also reported. Finally, the in vivo imaging of transplanted stem cells in mice by QDs emitting fluorescence in the near-infrared region, which can be detected by in vivo fluorescence imaging systems such as IVIS and SAI-1000, is described. The future prospects for stem cell imaging technology by QDs are also discussed.
与传统荧光探针相比，量子点（QDs）具有优异的荧光特性。因此，量子点的光学应用正在迅速扩展到分析化学的各个领域。在这篇综述文章中，我们回顾了量子点在再生医学，特别是干细胞移植治疗中的应用。报道了使用由半导体材料与化学物质、聚阳离子脂质体和细胞穿透肽组成的量子点对干细胞进行标记。此外，还报道了量子点标记对干细胞多能性的影响。最后，描述了通过近红外区域发射荧光的量子点对小鼠体内移植干细胞进行成像，这种成像可通过诸如IVIS和SAI - 1000等体内荧光成像系统进行检测。还讨论了量子点用于干细胞成像技术的未来前景。
