声敏性壳聚糖纳米液滴用于时空控制的前列腺癌细胞基因递释
Echogenic, Ultrasound-Sensitive Chitosan Nanodroplets for Spatiotemporally Controlled Gene Delivery to Prostate Cancer Cells.
机构信息
Department of Ultrasound, Qilu Hospital of Shandong University, Jinan, People's Republic of China.
出版信息
Int J Nanomedicine. 2021 Jan 15;16:421-432. doi: 10.2147/IJN.S286474. eCollection 2021.
PURPOSE
To synthesize echogenic chitosan/perfluorohexane nanodroplets (CNDs) for DKK-2 gene delivering in a spatiotemporally controlled manner in vitro.
METHODS
The characteristics, contrast-enhanced ultrasound imaging, DNA binding and DNase protection capacity, DKK-2 gene transfection and effects on LNCaP cells of these CNDs were investigated.
RESULTS
The obtained CNDs showed positive surface charges and could attract the genetic cargo with negative surface charges to form nanocomplexes. Agarose gel electrophoresis confirmed binding of the CNDs and pDNA. DKK-2 pDNA-loaded CNDs, in combination with ultrasound, ruptured and released DKK-2 pDNA, entering LNCaP cells through nano-scale pores in the cell membrane, which further reduced the proliferation of LNCaP cells.
CONCLUSION
These stable and safe CNDs may be a promising choice to achieve efficient ultrasound-mediated gene delivery to specific tissues in a spatiotemporally controlled manner.
目的
合成声敏壳聚糖/全氟己烷纳米液滴（CNDs），以便在体外时空可控方式下递呈 DKK-2 基因。
方法
研究了这些 CNDs 的特性、超声造影成像、DNA 结合和 DNA 酶保护能力、DKK-2 基因转染及其对 LNCaP 细胞的影响。
结果
得到的 CNDs 带正表面电荷，可吸引带负表面电荷的遗传货物形成纳米复合物。琼脂糖凝胶电泳证实了 CNDs 与 pDNA 的结合。载有 DKK-2 pDNA 的 CNDs 与超声联合使用可使 DKK-2 pDNA 破裂并释放，通过细胞膜上的纳米级孔隙进入 LNCaP 细胞，进一步降低了 LNCaP 细胞的增殖。
结论
这些稳定且安全的 CNDs 可能是一种有前途的选择，可实现高效的超声介导的基因递呈，以在时空可控的方式递呈至特定组织。
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