Student Research Committee, School of Medicine, Babol University of Medical Sciences, Babol, Iran.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Sci Rep. 2024 Aug 5;14(1):18117. doi: 10.1038/s41598-024-69220-x.
Diabetic nephropathy, characterized by inflammation and oxidative stress, poses a management challenge. This study investigates the effect of Polygonum hyrcanicum extract on diabetic nephropathy in alloxan-induced diabetic mice. In this experimental animal study, the P. hyrcanicum extract was prepared using continuous macerations. Thirty male Albino mice, divided into five groups, were induced with alloxan-induced diabetes. They received intraperitoneal injections of the plant extract (100 and 200 mg/kg) and metformin (300 mg/kg) for four weeks. Kidney and blood samples were collected to assess protein carbonyl, glutathione, lipid peroxidation, TNF-α and IL-6 levels. The amount of total flavonoid and phenolic content in the hydroalcoholic extract of P. hyrcanicum were 7.5 ± 0.3 mg of quercetin and 88.2 ± 1.3 mg gallic acid per gram of extract respectively. The antioxidant activity level of the hydroalcoholic extract was determined to be 1.78 ± 0.51 mM equivalent per gram of extract. Alloxan administration resulted in a significant reduction in glutathione levels and a significant increase in protein carbonyl, lipid peroxidation, TNF-α, and IL-6 levels. Hydroalcoholic extract of P. hyrcanicum effectively reduced oxidative stress markers and inflammatory cytokines (TNF-α, IL-6), indicating its potential in mitigating diabetic nephropathy. However, no significant difference in efficacy was observed between the 100 mg/kg and 200 mg/kg doses in terms of reducing these toxicities.
糖尿病肾病的特征为炎症和氧化应激，这给其治疗带来了挑战。本研究旨在探讨黑果枸杞提取物对四氧嘧啶诱导的糖尿病小鼠糖尿病肾病的影响。在这项实验动物研究中，采用连续浸渍法制备黑果枸杞提取物。将 30 只雄性白化小鼠分为五组，用四氧嘧啶诱导糖尿病。它们分别腹腔注射植物提取物（100 和 200mg/kg）和二甲双胍（300mg/kg），持续四周。采集肾脏和血液样本，以评估蛋白质羰基、谷胱甘肽、脂质过氧化、TNF-α和 IL-6 水平。黑果枸杞水醇提取物中总黄酮和总酚含量分别为 7.5±0.3mg 槲皮素和 88.2±1.3mg 没食子酸/克提取物。水醇提取物的抗氧化活性水平为 1.78±0.51mM 相当于每克提取物。四氧嘧啶给药导致谷胱甘肽水平显著降低，蛋白质羰基、脂质过氧化、TNF-α和 IL-6 水平显著升高。黑果枸杞水醇提取物可有效降低氧化应激标志物和炎症细胞因子（TNF-α、IL-6），表明其在减轻糖尿病肾病方面具有潜力。然而，在降低这些毒性方面，100mg/kg 和 200mg/kg 剂量之间的疗效没有显著差异。
