Seyedian Ramin, Shabankareh Fard Elham, Najafiasl Maryam, Assadi Majid, Zaeri Sasan
Department of Pharmacology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
Department of Environmental Health Engineering, Faculty of Health, Bushehr University of Medical Sciences, Bushehr, Iran.
Iran J Basic Med Sci. 2020 Dec;23(12):1590-1602. doi: 10.22038/ijbms.2020.41550.11078.
N-acetylcysteine (NAC) has gained attention recently in dermatology as a unique anti-oxidant. In light of progress in nanotechnological methods, it was hypothesized that loading NAC onto nanofibers would positively affect skin wound healing. The objective of this study was to fabricate NAC-loaded electrospun mats and test their effect on wound healing and .
Polyvinyl alcohol (PVA)-based mats loaded with NAC at three concentrations were electrospun and characterized in terms of physicochemical properties and drug release profile. Human fibroblast cells () and mouse full-thickness skin wounds () were treated with mats for 5 and 14 days, respectively. Wound area, tissue histopathology, fibroblast proliferation and cellular oxidative state were evaluated.
Mats containing 5% PVA/NAC showed thinner fibers with suitable physicochemical properties and a sustained drug release profile. PVA/NAC (5%) mats enhanced fibroblast proliferation and attachment . The mats resulted in significant wound closure with high levels of re-epithelialization and collagen fiber synthesis on day 14 post-surgery The mats also reduced granulation tissue and edematous stroma to a higher extent. These findings were accompanied by a significant decrease in tissue lipid peroxidation and higher superoxide dismutase activity, which may explain how NAC improved wound healing.
We propose an NAC-loaded nanofibrous mat that takes the advantage of a porous nanoscaffold structure to release NAC in a sustained manner. This mat may be a promising candidate for further clinical evaluation.
N-乙酰半胱氨酸（NAC）作为一种独特的抗氧化剂，近年来在皮肤科受到关注。鉴于纳米技术方法的进展，有人推测将NAC负载到纳米纤维上会对皮肤伤口愈合产生积极影响。本研究的目的是制备负载NAC的电纺垫，并测试其对伤口愈合的影响。
制备了三种浓度负载NAC的聚乙烯醇（PVA）基垫，对其理化性质和药物释放曲线进行了表征。分别用垫子处理人成纤维细胞和小鼠全层皮肤伤口5天和14天。评估伤口面积、组织组织病理学、成纤维细胞增殖和细胞氧化状态。
含5%PVA/NAC的垫子显示出较细的纤维，具有合适的理化性质和持续的药物释放曲线。PVA/NAC（5%）垫子增强了成纤维细胞的增殖和附着。术后14天，这些垫子导致伤口显著闭合，上皮再形成和胶原纤维合成水平较高。这些垫子还在更大程度上减少了肉芽组织和水肿性基质。这些发现伴随着组织脂质过氧化的显著降低和超氧化物歧化酶活性的提高，这可能解释了NAC如何改善伤口愈合。
我们提出了一种负载NAC的纳米纤维垫，它利用多孔纳米支架结构持续释放NAC。这种垫子可能是进一步临床评估的有希望的候选者。
