通过碱和有机溶剂对甘蔗渣进行顺序预处理，以提高木质素脱除率，并通过嵌合体和纤维二糖水解酶实现纤维素糖化，用于生物乙醇生产。
Sequential pretreatment of sugarcane bagasse by alkali and organosolv for improved delignification and cellulose saccharification by chimera and cellobiohydrolase for bioethanol production.
作者信息
Nath Priyanka, Maibam Premeshworii Devi, Singh Shweta, Rajulapati Vikky, Goyal Arun
机构信息
Carbohydrate Enzyme Biotechnology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039 India.
DBT PAN-IIT Center for Bioenergy, Indian Institute of Technology Guwahati, Guwahati, Assam India.
出版信息
3 Biotech. 2021 Feb;11(2):59. doi: 10.1007/s13205-020-02600-y. Epub 2021 Jan 11.
UNLABELLED
Sequential pretreatments for sugarcane bagasse (scb) by NaOH followed by organosolv under mild conditions were evaluated for cellulose recovery and dilignification. The best-optimized sequential pretreatment of scb was obtained at 10% (w/v) of raw scb loading at 1% (w/v) NaOH (50 °C, 2 h) followed by treatment with organosolv (85%, v/v phosphoric acid, 50 °C, 1 h) with chilled acetone. This sequentially pretreated scb showed cellulose recovery, 66.1% (w/w) and delignification, 83.2% (w/w). NaOH or organosolv pretreated scb showed lower cellulose recovery 47.4% (w/w) or 54.5% (w/w) with lower delignification, 61% (w/w) or 56% (w/w), respectively. Pretreated solid residue of sequentially pretreated scb was enzymatically saccharified by chimera (β-glucosidase and endoglucanase, GH1-L1-GH5-F194A) and cellobiohydrolase (CBH5A) cloned from . Enzymatic hydrolysate of best sequentially pretreated scb gave total reducing sugar (TRS) yield, 230 mg/g and glucose yield, 137 mg/g pretreated scb. Only organosolv pretreated scb gave TRS yield, 112.5 mg/g and glucose yield, 72 mg/g of pretreated scb. Thus, sequentially pretreated scb resulted in 37% higher enzymatic digestibility than only orgnaosolv pretreated scb. Higher enzymatic digestibility was supported by higher crystallinity index (45%) than those obtained with only organosolv pretreated (38%) or raw scb (25%). Field Emission Scanning Electron Microscope (FESEM) and Fourier-transform infrared (FT-IR) analyses showed enhanced cellulose exposure in sequentially pretreated scb. Preliminary investigation of bioethanol production at small scale by separate hydrolysis and fermentation (SHF) of enzymatic hydrolysate from best sequentially pretreated scb by gave maximum ethanol yield of 0.42 g/g of glucose.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s13205-020-02600-y.
未标记
评估了在温和条件下先用氢氧化钠对甘蔗渣进行预处理，然后用有机溶剂处理，以实现纤维素回收和脱木质素。在1%（w/v）氢氧化钠（50℃，2小时）处理10%（w/v）的原始甘蔗渣负载量，随后用有机溶剂（85%，v/v磷酸，50℃，1小时）加冷冻丙酮处理的条件下，获得了最佳优化的甘蔗渣顺序预处理。这种顺序预处理的甘蔗渣显示纤维素回收率为66.1%（w/w），脱木质素率为83.2%（w/w）。仅用氢氧化钠或有机溶剂预处理的甘蔗渣纤维素回收率较低，分别为47.4%（w/w）或54.5%（w/w），脱木质素率也较低，分别为61%（w/w）或56%（w/w）。顺序预处理的甘蔗渣的预处理固体残渣用从……克隆的嵌合体（β-葡萄糖苷酶和内切葡聚糖酶，GH1-L1-GH5-F194A）和纤维二糖水解酶（CBH5A）进行酶解糖化。最佳顺序预处理的甘蔗渣的酶解水解产物产生的总还原糖（TRS）产量为230毫克/克预处理甘蔗渣，葡萄糖产量为137毫克/克预处理甘蔗渣。仅用有机溶剂预处理的甘蔗渣产生的TRS产量为112.5毫克/克预处理甘蔗渣，葡萄糖产量为72毫克/克预处理甘蔗渣。因此，顺序预处理的甘蔗渣的酶解消化率比仅用有机溶剂预处理的甘蔗渣高37%。较高的酶解消化率得到了较高结晶度指数（45%）的支持，该指数高于仅用有机溶剂预处理（38%）或原始甘蔗渣（25%）的结晶度指数。场发射扫描电子显微镜（FESEM）和傅里叶变换红外光谱（FT-IR）分析表明，顺序预处理的甘蔗渣中纤维素的暴露增加。对最佳顺序预处理的甘蔗渣的酶解水解产物通过单独水解和发酵（SHF）进行小规模生物乙醇生产的初步研究表明，最大乙醇产量为0.42克/克葡萄糖。
补充信息
在线版本包含可在10.1007/s13205-020-02600-y获取的补充材料。
Zotero 插件