Premjet Duangporn, Premjet Siripong
Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand.
Department of Biology, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.
Polymers (Basel). 2025 Jan 21;17(3):266. doi: 10.3390/polym17030266.
The effective utilization of nonfood biomass for bioethanol production represents a promising strategy for sustainable energy development. Moreover, limited research has been conducted on broom grass () as a potential feedstock for bioethanol production, particularly regarding the effects of NaOH autoclave pretreatment on its enzymatic digestibility and fermentability. This study optimized sodium hydroxide (NaOH) pretreatment combined with autoclaving to enhance the enzymatic digestibility of broom grass biomass. The effects of NaOH concentration (1-4%) and temperature (110-130 °C) on biomass composition, structural features, and enzymatic hydrolysis were systematically evaluated. Pretreatment with 2% NaOH at 120 °C emerged as optimal, achieving 74.7% lignin removal and 93.2% glucan recovery, thereby significantly improving enzymatic hydrolysis efficiency (88.0%) and glucose recovery (33.3%). Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analyses revealed that these improvements were attributed to the increased surface porosity and the selective removal of amorphous components while maintaining cellulose crystallinity. The pretreated biomass hydrolysate exhibited excellent bioethanol production. Fermentation using TISTR 5339 achieved an 86.4% ethanol conversion rate, yielding 147 g of bioethanol per 1000 g of pretreated biomass and representing a 2.6-fold increase compared to untreated feedstock. These findings demonstrate the potential of the NaOH autoclave pretreatment in enhancing bioethanol production from broom grass biomass, aiding the advancement of sustainable and cost-effective lignocellulosic biorefinery processes. The utilization of broom grass for bioethanol production presents an opportunity to valorize this multifaceted plant and expand its potential beyond its traditional uses.
将非食用生物质有效用于生物乙醇生产是可持续能源发展的一项有前景的策略。此外，关于帚草作为生物乙醇生产潜在原料的研究有限，特别是关于氢氧化钠高压釜预处理对其酶解性和发酵性的影响。本研究优化了氢氧化钠（NaOH）预处理与高压灭菌相结合的方法，以提高帚草生物质的酶解性。系统评估了NaOH浓度（1-4%）和温度（110-130°C）对生物质组成、结构特征和酶水解的影响。在120°C下用2%NaOH预处理成为最佳方案，实现了74.7%的木质素去除率和93.2%的葡聚糖回收率，从而显著提高了酶水解效率（88.0%）和葡萄糖回收率（33.3%）。扫描电子显微镜（SEM）和X射线衍射（XRD）分析表明，这些改进归因于表面孔隙率增加以及在保持纤维素结晶度的同时选择性去除无定形成分。预处理后的生物质水解产物表现出优异的生物乙醇生产性能。使用TISTR 5339进行发酵，乙醇转化率达到86.4%，每1000克预处理生物质可产生147克生物乙醇，与未处理原料相比提高了2.6倍。这些发现证明了NaOH高压釜预处理在提高帚草生物质生物乙醇产量方面的潜力，有助于推进可持续且具有成本效益的木质纤维素生物精炼工艺。利用帚草生产生物乙醇为使这种多用途植物增值并拓展其传统用途之外的潜力提供了机会。
