School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu, Guangzhou 510006, People's Republic of China.
School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu, Guangzhou 510006, People's Republic of China; Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, PR China.
Bioresour Technol. 2020 Apr;301:122756. doi: 10.1016/j.biortech.2020.122756. Epub 2020 Jan 9.
Pretreatment process plays a key role in biofuel production from lignocellulosic feedstocks. A study on dilute NaOH pretreatment supplemented with HO under mild condition was conducted to overcome the recalcitrance of grass waste (GW). The optimized process could selectively increase lignin removal (73.2%), resulting in high overall recovery of holocellulose (73.8%) as well as high enzymolysis efficiency (83.5%) compared to HO or NaOH pretreatment. The analyses by Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) revealed that supplementary HO disrupted the structure of GW to facilitate the removal of lignin by NaOH, and exhibited synergistic effect on lignin removal and enzymolysis with dilute NaOH. Moreover, high titer of ethanol (100.7 g/L) was achieved by SSCF on 30% (w/v) pretreated GW loading. The present study suggests that the established synergistic pretreatment is a simple, efficient, and promising process for GW biorefinery.
预处理过程在木质纤维素原料生物燃料生产中起着关键作用。本研究采用温和条件下补充 HO 的稀 NaOH 预处理方法来克服草废料 (GW) 的抗降解性。与 HO 或 NaOH 预处理相比，优化后的工艺可以选择性地提高木质素去除率（73.2%），从而实现了全纤维素（73.8%）的高总体回收率和高酶解效率（83.5%）。扫描电子显微镜 (SEM)、X 射线衍射 (XRD) 和傅里叶变换红外光谱 (FT-IR) 的分析表明，补充的 HO 破坏了 GW 的结构，有利于 NaOH 去除木质素，并且与稀 NaOH 协同作用于木质素去除和酶解。此外，在 30%（w/v）预处理 GW 负载量下，通过同步糖化发酵 (SSCF) 可获得高浓度的乙醇（100.7 g/L）。本研究表明，所建立的协同预处理是一种简单、高效、有前景的 GW 生物炼制工艺。
