Wang Dan, Liu Yanmei, Li Qiang, Wang Lei, Liu Zhizhi
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China.
Central Laboratory of Shanghai Xuhui Central Hospital, Shanghai, China.
Mitochondrial DNA B Resour. 2016 Aug 21;1(1):566-568. doi: 10.1080/23802359.2016.1167638.
is a new record species of Gobiidae in China. It had been misidentified as for long time early before 2006. Here, the complete mitochondrial genome (mtDNA) sequences of the two species were first reported and analyzed comparably. The two genomes were both consisted of 13 protein-coding genes, 22 tRNA genes, two rRNA genes and a control region. Except for eight tRNA and ND6 genes, all other mitochondrial genes were encoded on the heavy strand. It was 16,496 bp and 16,803 bp, respectively, and had a 238 bp-gap between tRNAleu and ND5. There were high variations (10-19%) in the protein-coding genes. In the initiation condon and stop codons, the two fish also had tiny difference. Phylogenetic analysis showed that and first clustered together and then they constituted Gobiidae clade with other 12 fish. Whereas Odontobutidae and Rhyacichthyidae formed the sister group, then clustered with Eleotridae, and finally joined with Gobiidae, which is consist with previous phenotypic report. The study will contribute to the phylogenetic analysis of the Gobiidae and natural resources conservation of and .
是中国虾虎鱼科的一个新记录物种。在2006年之前很长一段时间里它一直被误认。在此，首次报道并比较分析了这两个物种的完整线粒体基因组（mtDNA）序列。这两个基因组均由13个蛋白质编码基因、22个tRNA基因、两个rRNA基因和一个控制区组成。除了8个tRNA和ND6基因外，所有其他线粒体基因都在重链上编码。它分别为16496 bp和16803 bp，并且在tRNAleu和ND5之间有一个238 bp的缺口。蛋白质编码基因存在高度变异（10 - 19%）。在起始密码子和终止密码子方面，这两种鱼也有细微差异。系统发育分析表明，[物种名称1]和[物种名称2]首先聚类在一起，然后它们与其他12种鱼类构成虾虎鱼科分支。而塘鳢科和吻虾虎鱼科形成姐妹群，然后与弹涂鱼科聚类，最后与虾虎鱼科合并，这与之前的表型报告一致。该研究将有助于虾虎鱼科的系统发育分析以及[物种名称1]和[物种名称2]的自然资源保护。
